Detailed information of TA system TA189538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1020430..1021049
• Replicon: chromosome
• Accession: NZ_CP069073
• Organism: Mycobacterium tuberculosis strain N0054

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN140_RS04860
• Protein ID: WP_003404726.1
• Coordinates: 1020615..1021049 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: JN140_RS04855
• Protein ID: WP_003898641.1
• Coordinates: 1020430..1020609 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN140_RS04840	1015903..1017483	+	1581	WP_003909313.1	serine hydrolase	-
JN140_RS04845	1017480..1019873	+	2394	WP_016719446.1	cation-translocating P-type ATPase	-
JN140_RS04850	1020146..1020304	+	159	WP_003404720.1	hypothetical protein	-
JN140_RS04855	1020430..1020609	+	180	WP_003898641.1	antitoxin	Antitoxin
JN140_RS04860	1020615..1021049	+	435	WP_003404726.1	SRPBCC family protein	Toxin
JN140_RS04865	1021147..1021920	+	774	WP_003404735.1	VOC family protein	-
JN140_RS04870	1021985..1022434	+	450	WP_003404738.1	hypothetical protein	-
JN140_RS04875	1022569..1022799	-	231	WP_003898642.1	hypothetical protein	-
JN140_RS04880	1022966..1024474	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
JN140_RS04885	1024476..1025714	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T189538 WP_003404726.1 NZ_CP069073:1020615-1021049 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T189538 NZ_CP090870:c512244-511939 [Staphylococcus aureus]
ATGGATTGGGAAATTAAAAGTTTATTTAGCGATTTAAAGTTGTTGAAAGATAGTTTTGAGGATTTAAAGGATAGTAATGG
TTGGCATTTTGATGAGCTATATCCATATGAACCAAATCATGTCTTAAATAAAGATGAATTAATTGGAGAGGGATTTTCTT
ATCATGAGAGACGTATTCACAATAATCAAATGTTTGATTTATTCCATCTCTATATAGAGCAGTTCGATAATATTATCGAA
AAGTTTTATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCATAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT189538 WP_003898641.1 NZ_CP069073:1020430-1020609 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT189538 NZ_CP090870:c512456-512247 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCAGAAAAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5