Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 723005..723669 Replicon chromosome
Accession NZ_CP069073
Organism Mycobacterium tuberculosis strain N0054

Toxin (Protein)


Gene name vapC Uniprot ID P96917
Locus tag JN140_RS03335 Protein ID WP_003403246.1
Coordinates 723262..723669 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WF18
Locus tag JN140_RS03330 Protein ID WP_003403244.1
Coordinates 723005..723265 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN140_RS03300 718259..719350 + 1092 WP_003900977.1 galactokinase -
JN140_RS03305 719680..720246 + 567 Protein_655 hypothetical protein -
JN140_RS03310 720350..721297 + 948 WP_003403232.1 DUF732 domain-containing protein -
JN140_RS03315 721390..721644 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 -
JN140_RS03320 721644..722039 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 -
JN140_RS03325 722133..722873 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
JN140_RS03330 723005..723265 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JN140_RS03335 723262..723669 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin Toxin
JN140_RS03340 723741..724892 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
JN140_RS03345 724985..726712 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-57)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14376.37 Da        Isoelectric Point: 4.3568

>T189533 WP_003403246.1 NZ_CP069073:723262-723669 [Mycobacterium tuberculosis]
VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAAR
MWARLRIHLAESGRRVRINDLWIAAVAASRALPVITQDDDFAALDGAASVEIIRV

Download         Length: 408 bp

>T189533 NZ_CP090838:c3497123-3496773 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9492.84 Da        Isoelectric Point: 11.9108

>AT189533 WP_003403244.1 NZ_CP069073:723005-723265 [Mycobacterium tuberculosis]
MSEVASRELRNDTAGVLRRVRAGEDVTITVSGRPVAVLTPVRPRRRRWLSKTEFLSRLRGAQADPGLRNDLAVLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT189533 NZ_CP090838:c3497410-3497129 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3DBO

Antitoxin

Source ID Structure
PDB 3DBO
AlphaFold DB A0A7U4BSE4

References