Detailed information of TA system TA189518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3753734..3754263
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: JN141_RS17740
• Protein ID: WP_003417760.1
• Coordinates: 3754006..3754263 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: JN141_RS17735
• Protein ID: WP_003417757.1
• Coordinates: 3753734..3754009 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS17700	3750307..3750996	-	690	WP_003917774.1	BBE domain-containing protein	-
JN141_RS17705	3751183..3751554	-	372	WP_003417739.1	FAD-binding protein	-
JN141_RS17710	3751452..3751670	-	219	WP_157132559.1	hypothetical protein	-
JN141_RS17715	3751697..3751957	-	261	WP_003417741.1	hypothetical protein	-
JN141_RS17720	3752072..3752461	+	390	WP_003417745.1	DUF732 domain-containing protein	-
JN141_RS17725	3752475..3752768	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
JN141_RS17730	3752765..3753610	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
JN141_RS17735	3753734..3754009	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
JN141_RS17740	3754006..3754263	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
JN141_RS17745	3754305..3755495	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
JN141_RS17750	3755612..3755980	+	369	WP_003417765.1	FHA domain-containing protein	-
JN141_RS17755	3755977..3756528	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
JN141_RS17760	3756535..3757116	-	582	WP_003417769.1	ATP/GTP-binding protein	-
JN141_RS17765	3757097..3757465	-	369	WP_003417772.1	DUF742 domain-containing protein	-
JN141_RS17770	3757443..3757835	-	393	WP_003417776.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T189518 WP_003417760.1 NZ_CP069072:3754006-3754263 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T189518 NZ_CP090834:c505841-505458 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT189518 WP_003417757.1 NZ_CP069072:3753734-3754009 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT189518 NZ_CP090834:c506083-505841 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94