Detailed information of TA system TA189515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3182222..3182909
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: JN141_RS15180
• Protein ID: WP_003414624.1
• Coordinates: 3182466..3182909 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: JN141_RS15175
• Protein ID: WP_003414620.1
• Coordinates: 3182222..3182479 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS15155	3177542..3178396	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
JN141_RS15160	3178452..3179615	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
JN141_RS15165	3179632..3180846	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
JN141_RS15170	3180854..3182095	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
JN141_RS15175	3182222..3182479	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
JN141_RS15180	3182466..3182909	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN141_RS15185	3182989..3183651	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
JN141_RS15190	3183747..3183938	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
JN141_RS15195	3184270..3186018	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
JN141_RS15200	3186114..3186695	+	582	WP_003414644.1	fasciclin domain-containing protein	-
JN141_RS15205	3186795..3187061	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T189515 WP_003414624.1 NZ_CP069072:3182466-3182909 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T189515 NZ_CP090796:c3800482-3800072 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTGGTGTTTGATAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGGGATCTGCGCCGGCTAATCGTGCAACAGCCAA
CGCAGGAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT189515 WP_003414620.1 NZ_CP069072:3182222-3182479 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT189515 NZ_CP090796:c3800729-3800463 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0