Detailed information of TA system TA189513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3135678..3136282
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: JN141_RS14955
• Protein ID: WP_003414492.1
• Coordinates: 3135678..3136070 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: JN141_RS14960
• Protein ID: WP_003414495.1
• Coordinates: 3136067..3136282 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS14925	3130828..3131616	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
JN141_RS14930	3131950..3132495	-	546	WP_003913758.1	DUF1802 family protein	-
JN141_RS14935	3132767..3133651	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JN141_RS14940	3133654..3134541	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
JN141_RS14945	3134846..3135391	-	546	WP_003899500.1	DUF1802 family protein	-
JN141_RS14950	3135388..3135657	-	270	WP_003414489.1	DUF2277 family protein	-
JN141_RS14955	3135678..3136070	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN141_RS14960	3136067..3136282	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JN141_RS14965	3136329..3137078	+	750	WP_003414497.1	enoyl-CoA hydratase	-
JN141_RS14970	3137157..3138239	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
JN141_RS14975	3138232..3139542	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
JN141_RS14980	3139545..3140372	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
JN141_RS14985	3140369..3141280	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T189513 WP_003414492.1 NZ_CP069072:c3136070-3135678 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T189513 NZ_CP090796:c2237125-2236640 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACCTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGGAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCAGTTGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT189513 WP_003414495.1 NZ_CP069072:c3136282-3136067 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT189513 NZ_CP090796:c2237379-2237113 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCGGCCATAGAAAAACTGCTG
GCAAGGAAACCACAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8