Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3109284..3109854 Replicon chromosome
Accession NZ_CP069072
Organism Mycobacterium tuberculosis strain N1216

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JN141_RS14810 Protein ID WP_003414166.1
Coordinates 3109284..3109640 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JN141_RS14815 Protein ID WP_003901465.1
Coordinates 3109624..3109854 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN141_RS14790 3104736..3106424 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
JN141_RS14795 3106428..3106754 - 327 WP_003414157.1 hypothetical protein -
JN141_RS14800 3106927..3107514 + 588 WP_003914429.1 DUF3558 family protein -
JN141_RS14805 3107533..3109182 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
JN141_RS14810 3109284..3109640 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JN141_RS14815 3109624..3109854 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
JN141_RS14820 3109897..3110940 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
JN141_RS14825 3110939..3111406 + 468 WP_003414177.1 DUF1778 domain-containing protein -
JN141_RS14830 3111582..3111836 - 255 WP_003917684.1 hypothetical protein -
JN141_RS14835 3111984..3112388 + 405 WP_009938577.1 hypothetical protein -
JN141_RS14840 3112385..3112576 + 192 WP_003414184.1 hypothetical protein -
JN141_RS14845 3112793..3113053 + 261 Protein_2928 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T189511 WP_003414166.1 NZ_CP069072:c3109640-3109284 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T189511 NZ_CP090796:c1101730-1101512 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT189511 WP_003901465.1 NZ_CP069072:c3109854-3109624 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT189511 NZ_CP090796:c1102132-1101758 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References