Detailed information of TA system TA189509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3069349..3070028
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JN141_RS14580
• Protein ID: WP_003414059.1
• Coordinates: 3069349..3069765 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN141_RS14585
• Protein ID: WP_003414061.1
• Coordinates: 3069762..3070028 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS14555	3065401..3066303	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN141_RS14560	3066372..3067124	-	753	WP_031650932.1	FAD-dependent thymidylate synthase	-
JN141_RS14570	3067368..3067643	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN141_RS14575	3067640..3069262	-	1623	WP_003906897.1	class I SAM-dependent DNA methyltransferase	-
JN141_RS14580	3069349..3069765	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JN141_RS14585	3069762..3070028	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN141_RS14590	3070054..3070449	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JN141_RS14595	3070446..3070715	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN141_RS14600	3070725..3071819	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN141_RS14605	3071816..3072235	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN141_RS14610	3072234..3072308	+	75	Protein_2881	hypothetical protein	-
JN141_RS14615	3072309..3072788	-	480	WP_003414073.1	dihydrofolate reductase	-
JN141_RS14620	3072859..3073659	-	801	WP_003911953.1	thymidylate synthase	-
JN141_RS14625	3073815..3074552	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T189509 WP_003414059.1 NZ_CP069072:c3069765-3069349 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T189509 NZ_CP090796:c299746-299444 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTGGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189509 WP_003414061.1 NZ_CP069072:c3070028-3069762 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189509 NZ_CP090796:c299437-299150 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64