Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2930452..2931166 Replicon chromosome
Accession NZ_CP069072
Organism Mycobacterium tuberculosis strain N1216

Toxin (Protein)


Gene name vapC Uniprot ID G0TQK0
Locus tag JN141_RS13755 Protein ID WP_003413460.1
Coordinates 2930726..2931166 (+) Length 147 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ20
Locus tag JN141_RS13750 Protein ID WP_003413456.1
Coordinates 2930452..2930739 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN141_RS13715 2925874..2926119 + 246 WP_003413429.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JN141_RS13720 2926116..2926520 + 405 WP_003899389.1 type II toxin-antitoxin system VapC family toxin -
JN141_RS13725 2926737..2927357 + 621 WP_003413441.1 DUF4178 domain-containing protein -
JN141_RS13730 2927368..2927862 + 495 WP_003413444.1 DUF2617 family protein -
JN141_RS13735 2927859..2928290 + 432 WP_031650926.1 DUF4247 domain-containing protein -
JN141_RS13740 2928315..2928773 + 459 WP_003413451.1 DUF350 domain-containing protein -
JN141_RS13745 2928770..2930341 + 1572 WP_003899392.1 polyamine aminopropyltransferase -
JN141_RS13750 2930452..2930739 + 288 WP_003413456.1 antitoxin VapB41 Antitoxin
JN141_RS13755 2930726..2931166 + 441 WP_003413460.1 type II toxin-antitoxin system VapC family toxin Toxin
JN141_RS13760 2931187..2931942 - 756 WP_003413464.1 YebC/PmpR family DNA-binding transcriptional regulator -
JN141_RS13765 2932075..2932671 - 597 WP_003413465.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
JN141_RS13770 2932679..2933524 - 846 WP_003413466.1 acyl-CoA thioesterase II -
JN141_RS13775 2933553..2934452 - 900 WP_031650927.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
JN141_RS13780 2934580..2935254 + 675 WP_003413471.1 pyridoxamine 5'-phosphate oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-146)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 147 a.a.        Molecular weight: 16026.38 Da        Isoelectric Point: 6.8599

>T189507 WP_003413460.1 NZ_CP069072:2930726-2931166 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Download         Length: 441 bp

>T189507 NZ_CP090793:c135188-134664 [Enterobacter hormaechei]
GTGGCCAACCTGACCATTGAGATGTTTTCAGAAGAGGCTGTATATGATTTCTCATGCTTCGACTGCGGAGAAACGTCTCT
CAATGATTTTCTTAACAATCGCCTCGCCCAGCAACACAGCGGGCGTATATTACGCGGATATCTGCTTCTGACGAGAGATC
CGATACCGAAAGTGATGGGGTTTTACACGTTGTCCGGGAGCTGCTTTGCAAGAAACACGCTTCCTTCCAACACTCAACAG
CGTAAAGTCCCCTATTCCGACGCCCCAAGCGTTACGCTCGGTCGTCTGGCAATAGACAAAAGTATCCAGCGACAAGGGTA
TGGGGAAACGCTGGTGGCACATGCGATGAAAGTGGTATACCGGGCTTCCCTGGCCGTTGGGATCTACGCTTTGTTTGTTG
ACGCCAAAAATGAGAATGCGATGCGGTTTTATAAGCAGTTAGGCTTTACCCCTCTTACGGGTGATAACGCCAATTCGCTG
TTCTATCCCACAAAAGGCATTGAGAAGTTGTTTGGTGGAAAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10453.98 Da        Isoelectric Point: 7.2797

>AT189507 WP_003413456.1 NZ_CP069072:2930452-2930739 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Download         Length: 288 bp

>AT189507 NZ_CP090793:c135461-135192 [Enterobacter hormaechei]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACAATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGTCGCC
TGATTCTTAGCGAAGAGTCATGGAATATAGTGATGGATGCCATCAGTAACCCGCCTGCACCGAATGATAGGCTGAAACGA
GCTGCCGAGCGTCTACAAAGCATGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQK0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BWB8

References