Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2870112..2870749 Replicon chromosome
Accession NZ_CP069072
Organism Mycobacterium tuberculosis strain N1216

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN141_RS13485 Protein ID WP_003413180.1
Coordinates 2870112..2870507 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN141_RS13490 Protein ID WP_003413183.1
Coordinates 2870504..2870749 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN141_RS13445 2865514..2866726 + 1213 Protein_2653 alpha/beta hydrolase family protein -
JN141_RS13450 2866853..2867512 + 660 WP_003900846.1 LppA family lipoprotein -
JN141_RS13455 2867509..2868171 + 663 WP_003900848.1 LppA family lipoprotein -
JN141_RS13460 2868168..2868446 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN141_RS13465 2868539..2868952 + 414 WP_003413164.1 PIN domain nuclease -
JN141_RS13470 2868991..2869248 + 258 WP_003413167.1 CopG family transcriptional regulator -
JN141_RS13475 2869245..2869622 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN141_RS13480 2869638..2870012 - 375 WP_003413177.1 hypothetical protein -
JN141_RS13485 2870112..2870507 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN141_RS13490 2870504..2870749 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN141_RS13495 2871160..2871579 - 420 WP_003413190.1 A24 family peptidase -
JN141_RS13500 2871591..2872400 - 810 WP_003413193.1 shikimate dehydrogenase -
JN141_RS13505 2872397..2873650 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN141_RS13510 2873643..2874155 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189506 WP_003413180.1 NZ_CP069072:c2870507-2870112 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189506 NZ_CP090792:c4921116-4920745 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189506 WP_003413183.1 NZ_CP069072:c2870749-2870504 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189506 NZ_CP090792:c4921361-4921119 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAGCCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References