Detailed information of TA system TA189505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2868991..2869622
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: JN141_RS13475
• Protein ID: WP_003413174.1
• Coordinates: 2869245..2869622 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: JN141_RS13470
• Protein ID: WP_003413167.1
• Coordinates: 2868991..2869248 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS13440	2864904..2865218	+	315	WP_009937839.1	hypothetical protein	-
JN141_RS13445	2865514..2866726	+	1213	Protein_2653	alpha/beta hydrolase family protein	-
JN141_RS13450	2866853..2867512	+	660	WP_003900846.1	LppA family lipoprotein	-
JN141_RS13455	2867509..2868171	+	663	WP_003900848.1	LppA family lipoprotein	-
JN141_RS13460	2868168..2868446	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
JN141_RS13465	2868539..2868952	+	414	WP_003413164.1	PIN domain nuclease	-
JN141_RS13470	2868991..2869248	+	258	WP_003413167.1	CopG family transcriptional regulator	Antitoxin
JN141_RS13475	2869245..2869622	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN141_RS13480	2869638..2870012	-	375	WP_003413177.1	hypothetical protein	-
JN141_RS13485	2870112..2870507	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JN141_RS13490	2870504..2870749	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JN141_RS13495	2871160..2871579	-	420	WP_003413190.1	A24 family peptidase	-
JN141_RS13500	2871591..2872400	-	810	WP_003413193.1	shikimate dehydrogenase	-
JN141_RS13505	2872397..2873650	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
JN141_RS13510	2873643..2874155	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T189505 WP_003413174.1 NZ_CP069072:2869245-2869622 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T189505 NZ_CP090792:c4797631-4797446 [Enterobacter hormaechei]
GTGAAAAGTGCGGATATCATTGCTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGAAGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTAACCGTTCCGCATCCTAAAAAGGACATTAAACCGGGCACTCTCGCACAGATCT
GGCGACAGGCGGGCATTAAACACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT189505 WP_003413167.1 NZ_CP069072:2868991-2869248 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT189505 NZ_CP090792:c4797431-4797024 [Enterobacter hormaechei]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGCGGTTTCTTCCCCGACGTTCCGGGATGTTTTTT
TGCGGGGGATTCACTTGATGACGCTTTTCAGGATGCACGTGATGCGCTCACGGCGCACTTCGAAGCGCTGTTTGAAATGG
ATGAAGCATTGCCTCTCCCGGGTAGTGTCGAAGTGCACCTTGAAAATCAACCTGACGACTTCACAGGCGGGCAATGGTTG
CTGGTGGATATTAATATGAAGCAATTCGACGGCAGAGCCGAACGCATCAACATCACGATGCCTCGACGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCTCAATTCGGCAATCGAAGCGCATTTCTGGCCGAGGCAGCACGTCGCGTGCTGC
CGCGGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5