Detailed information of TA system TA189503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2854651..2855291
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JN141_RS13380
• Protein ID: WP_003412970.1
• Coordinates: 2854651..2855070 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JN141_RS13385
• Protein ID: WP_003412975.1
• Coordinates: 2855067..2855291 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS13350	2850236..2850958	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JN141_RS13355	2851475..2851702	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
JN141_RS13360	2851699..2852100	+	402	WP_003412963.1	PIN domain-containing protein	-
JN141_RS13365	2852135..2853055	-	921	WP_003412965.1	restriction endonuclease	-
JN141_RS13370	2853396..2853602	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN141_RS13375	2853700..2854650	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
JN141_RS13380	2854651..2855070	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN141_RS13385	2855067..2855291	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JN141_RS13390	2855322..2858165	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN141_RS13395	2858237..2858638	-	402	WP_003412981.1	hypothetical protein	-
JN141_RS13400	2858638..2859108	-	471	WP_003899364.1	transcription antitermination factor NusB	-
JN141_RS13405	2859111..2859674	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189503 WP_003412970.1 NZ_CP069072:c2855070-2854651 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189503 NZ_CP090792:c4022060-4021650 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCCTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGCGCGCCGTGGATGCTTAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT189503 WP_003412975.1 NZ_CP069072:c2855291-2855067 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189503 NZ_CP090792:c4022307-4022041 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09