Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2252195..2252821 Replicon chromosome
Accession NZ_CP069072
Organism Mycobacterium tuberculosis strain N1216

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag JN141_RS10565 Protein ID WP_003410075.1
Coordinates 2252423..2252821 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag JN141_RS10560 Protein ID WP_003911750.1
Coordinates 2252195..2252422 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN141_RS10550 2250234..2250578 - 345 WP_003410065.1 ferredoxin family protein -
JN141_RS10555 2250767..2251936 - 1170 WP_003899126.1 ATP-binding protein -
JN141_RS10560 2252195..2252422 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JN141_RS10565 2252423..2252821 + 399 WP_003410075.1 PIN domain nuclease Toxin
JN141_RS10570 2253004..2253435 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
JN141_RS10575 2253536..2253970 + 435 WP_003914358.1 DUF1398 domain-containing protein -
JN141_RS10580 2254407..2254586 - 180 Protein_2088 hypothetical protein -
JN141_RS10585 2254674..2255294 + 621 WP_003410086.1 IS110 family transposase -
JN141_RS10590 2255248..2255838 + 591 WP_003899131.1 IS110 family transposase -
JN141_RS10595 2255966..2257221 - 1256 Protein_2091 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T189497 WP_003410075.1 NZ_CP069072:2252423-2252821 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T189497 NZ_CP090792:c1200359-1200141 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT189497 WP_003911750.1 NZ_CP069072:2252195-2252422 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT189497 NZ_CP090792:c1200761-1200387 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References