Detailed information of TA system TA189495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 2226889..2227787
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: JN141_RS10440
• Protein ID: WP_003410001.1
• Coordinates: 2226889..2227449 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: JN141_RS10445
• Protein ID: WP_003410003.1
• Coordinates: 2227446..2227787 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS10410	2222058..2222711	-	654	WP_003409976.1	cutinase Cfp21	-
JN141_RS10415	2222826..2223056	+	231	WP_003409980.1	DUF167 domain-containing protein	-
JN141_RS10420	2223141..2224052	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
JN141_RS10425	2224161..2224760	+	600	WP_003409989.1	L-lysine exporter	-
JN141_RS10430	2225176..2225604	+	429	WP_003409992.1	cellulose-binding protein	-
JN141_RS10435	2225830..2226369	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
JN141_RS10440	2226889..2227449	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
JN141_RS10445	2227446..2227787	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
JN141_RS10450	2227873..2228130	+	258	WP_003410006.1	hypothetical protein	-
JN141_RS10455	2228031..2228366	-	336	WP_003410009.1	dehydrogenase	-
JN141_RS10460	2228455..2228799	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
JN141_RS10465	2228793..2229041	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
JN141_RS10470	2229141..2231456	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
JN141_RS10475	2231453..2231725	-	273	WP_003410017.1	DUF1490 family protein	-
JN141_RS10480	2231778..2232134	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T189495 WP_003410001.1 NZ_CP069072:c2227449-2226889 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T189495 NZ_CP090790:368109-368393 [Bifidobacterium longum]
ATGAGCACCGAAATCACCGACTACGACACCAGCGAATACCTCGAAAACGAACAGGACATCATCGCCTACCTCAACGCCAT
AGCCGAATACGACGACCCCGCACTCATGCAGGCCGCACTCGGCAACGTCGCCAAGGCTCGAGGCATGACCCAGATCGCCA
AGGACGCGGGCGTGGGGCGCGAAAGCCTCTACAAAAGCCTCAGCAAGGACGGAAACCCCAGCTTCCAGACCATCGCCAAG
GTAATCCACGCCCTCGGCGGACGCCTCACCATCCAAGCCGCCTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT189495 WP_003410003.1 NZ_CP069072:c2227787-2227446 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT189495 NZ_CP090790:367807-368112 [Bifidobacterium longum]
ATGGAAATCAAGCAAACCGCCGAATACCGCAAGTGGTTCAAGAAACTCAGGAACCGCGAGGCGAAAGCCGCCATCCAAGC
CCGGCTCGACGCCTGCAAGCTCGCCGGCAGGCCGTTCGGCGACATCAAACCCGTGGGAGGCCCGGTCAGCGAGATGCGGT
TCCACATCGGAGCCGGATACCGCGTCTACTTCACCACGCGCGGCAACGTGCTCATGCTGCTGCTCGCAGGCGGCGACAAA
AGCACCCAGCAGACCGACATCAAACAAGCCCACGCCATACTCGACGACTACAAGGAGCAGCAATGA

Structures

Toxin

• PDB: 6FKG

Antitoxin

• AlphaFold DB: A0A7U4FAE4