Detailed information of TA system TA189491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2188790..2189493
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JN141_RS10185
• Protein ID: WP_003409778.1
• Coordinates: 2188790..2189119 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JN141_RS10190
• Protein ID: WP_003409780.1
• Coordinates: 2189116..2189493 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS10165	2185173..2186243	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
JN141_RS10170	2186240..2186755	+	516	WP_003409718.1	flavin reductase family protein	-
JN141_RS10175	2186752..2187813	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JN141_RS10180	2187810..2188580	+	771	WP_003409775.1	SDR family oxidoreductase	-
JN141_RS10185	2188790..2189119	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JN141_RS10190	2189116..2189493	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JN141_RS10195	2189490..2190080	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
JN141_RS10200	2190135..2191499	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
JN141_RS10205	2191654..2192106	-	453	WP_003899095.1	lipoprotein	-
JN141_RS10210	2192170..2192571	+	402	WP_003409869.1	hypothetical protein	-
JN141_RS10215	2192564..2192746	-	183	WP_003409870.1	hypothetical protein	-
JN141_RS10220	2192860..2193210	-	351	WP_003899096.1	hypothetical protein	-
JN141_RS10225	2193221..2194123	-	903	WP_003899097.1	hypothetical protein	-
JN141_RS10230	2194144..2194335	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T189491 WP_003409778.1 NZ_CP069072:c2189119-2188790 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T189491 NZ_CP090788:c457472-457134 [Burkholderia vietnamiensis]
ATGGTTGACATATCGAGTAACGTGCCTTACAGTGCTCCGACGTACAGCATCAGAACGACCGAAGTCTTCGACACATGGTT
CGCCGGTCTGCAGGATCGCGTCGCACGGCGGCGCATCCAGGCCCGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACT
GGAAGTCGGCCGGCTCCCCGGTCGTCGAGATGCGTATCGACCACGGCCCCGGCTATCGCGTCTACTACGTCAGGCGCGCG
ACGATCTGGGTGATCCTGCTCTGCGGCGGCGACAAGTCGACGCAACAGGCAGACATTCGCACCGCGCACGCAATGCTCGG
GCGGCTCGACCTGGAGTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT189491 WP_003409780.1 NZ_CP069072:c2189493-2189116 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT189491 NZ_CP090788:c457130-456831 [Burkholderia vietnamiensis]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGCCCACCTCGAAACCGACGACGACATCGCCGACTATTTCGACGC
GTGCCTGCAGGAAGGCGGCGACGACCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCGCGCGCGCGCGGCATGTCGC
AGGTCGCCCGCGACGCCGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACC
ATCCTCAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCGCGCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8