Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1939275..1939888 Replicon chromosome
Accession NZ_CP069072
Organism Mycobacterium tuberculosis strain N1216

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag JN141_RS09060 Protein ID WP_003408465.1
Coordinates 1939275..1939664 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag JN141_RS09065 Protein ID WP_003408469.1
Coordinates 1939661..1939888 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN141_RS09025 1934904..1935818 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
JN141_RS09030 1935821..1936651 + 831 WP_003916363.1 cyclase family protein -
JN141_RS09035 1936651..1937001 + 351 WP_003898986.1 cupin domain-containing protein -
JN141_RS09040 1937054..1937872 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
JN141_RS09045 1937886..1938665 + 780 WP_003408460.1 IclR family transcriptional regulator -
JN141_RS09055 1939096..1939227 + 132 Protein_1783 IS3 family transposase -
JN141_RS09060 1939275..1939664 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
JN141_RS09065 1939661..1939888 - 228 WP_003408469.1 antitoxin Antitoxin
JN141_RS09070 1940106..1941590 + 1485 WP_003408473.1 biotin carboxylase -
JN141_RS09075 1941587..1942834 + 1248 WP_003408476.1 serine hydrolase -
JN141_RS09080 1942877..1943296 - 420 WP_003408483.1 hypothetical protein -
JN141_RS09085 1943286..1943996 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T189489 WP_003408465.1 NZ_CP069072:c1939664-1939275 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T189489 NZ_CP090786:c3198675-3198274 [Burkholderia vietnamiensis]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTACTTGATGAAGAGCCAGCCCGAGCAAGTGGCGAAGCGATTTGC
GCAGTGCTACACGGGCGACGTCGTGATGTCGGCGATCACGTATGCCGCGCTGGAGTACGGCGTCACCGCTTGCGCGGCGC
CCGCGCGTGAGCGCCGCCATCTTGCGGCGCTGATCGAGGATATTCCCGTCGCGCCGTTCGACGCCGCGGCCGCGCAAGCA
TATGGGCCCGTGCGTGACGCAACGCGCGCATGGAAGAAGGATCATCTCGACAAACTGATCGCCGCTCATGCGGTGTCGCT
CGATGTCGTGCTGGTGACGAATGACGAGCGCGATTTCACGAGCTATCCGGGATTGCGGCTGGAGAACTGGCTGAACGATT
AG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT189489 WP_003408469.1 NZ_CP069072:c1939888-1939661 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT189489 NZ_CP090786:c3198902-3198675 [Burkholderia vietnamiensis]
ATGCATACCACGAGGGTATTTCGGAACGGCAATTCGCAGGCCGTGCGAATTCCTGCGGATCTCGCTTATGAGCGCAGCGA
TATCGAGCTCGAGATCGAGCGCATCGGCGACGAGATCCGCATCCGGCCGGCGCGACGGCCGTTGACGCAGGTGCTCGAGA
AGTTCGCGCCCGATTTCATGGCGGAAGGGCGTGGCGACCACGAGCAGGCCGAACGCGAGGGGTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References