Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1689720..1690336 Replicon chromosome
Accession NZ_CP069072
Organism Mycobacterium tuberculosis strain N1216

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag JN141_RS07975 Protein ID WP_003407593.1
Coordinates 1690019..1690336 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag JN141_RS07970 Protein ID WP_003900349.1
Coordinates 1689720..1690022 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN141_RS07960 1685606..1687453 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
JN141_RS07965 1687454..1689706 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
JN141_RS07970 1689720..1690022 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
JN141_RS07975 1690019..1690336 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
JN141_RS07980 1690333..1691337 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
JN141_RS07985 1691390..1692679 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
JN141_RS07990 1692752..1693369 - 618 WP_003407606.1 class I SAM-dependent methyltransferase -
JN141_RS07995 1693583..1693795 - 213 WP_003898900.1 dodecin family protein -
JN141_RS08000 1693856..1694254 + 399 WP_003900351.1 hypothetical protein -
JN141_RS08005 1694299..1695327 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T189487 WP_003407593.1 NZ_CP069072:1690019-1690336 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T189487 NZ_CP090782:c3321555-3321154 [Burkholderia multivorans]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTATCTGATGAAGAACCAGCCGGAGCAGGTCGCGCGGCGTTTCGC
GCAGTGCTACACGGGGGACGTCGTCATGTCGGCGATCACCTATGCGGAACTCGAGTACGGCGTGACGCTATGCACCAATC
CTGCCCGCGAACGCCGCAATCTCGCCGCGCTGATCGAGGATATCCCGGTGGCGCCGTTCGACGTTGCGGCTGCGCAGGCA
TACGGCCCCATTCGAGAGGCGACGCGCGAACGCAAGAAGGATCATCTCGACAAGCTGATCGGCGCGCACGCCGTATCGCT
CGACGTCGTGCTGGTGACCAACAACGAGCGTGATTTCGCGAGTTATCCGGGCTTGAGGCTGGAGAACTGGCTGATCGGCT
AG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT189487 WP_003900349.1 NZ_CP069072:1689720-1690022 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT189487 NZ_CP090782:c3321791-3321555 [Burkholderia multivorans]
ATGCATACCACCCGGGTCTTCCGGAATGGCAACTCGCAGGCGGTGCGGATTCCTGCGGATCTTGCTTATGAGCGCAGCGA
TATCGAACTCGAAATCGAGCGTGTCGGTGACGAAATTCGCATCCGGCCTGCGCGGCGGCCGCTGACCGGCGTGCTCGGCA
AGTTCGCGAAATTCGGGCCCGATTTCATGGCCGAGGGGCGCGGCGATCAGGAACAGGCCGAGCGCGAGGGTTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References