Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1577282..1577937 Replicon chromosome
Accession NZ_CP069072
Organism Mycobacterium tuberculosis strain N1216

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA6
Locus tag JN141_RS07475 Protein ID WP_003898861.1
Coordinates 1577282..1577683 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag JN141_RS07480 Protein ID WP_003407272.1
Coordinates 1577680..1577937 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN141_RS07460 1572756..1574141 - 1386 WP_003407260.1 cytochrome P450 -
JN141_RS07465 1574219..1575253 + 1035 WP_003407264.1 AraC family transcriptional regulator -
JN141_RS07470 1575299..1577027 - 1729 Protein_1468 PE family protein -
JN141_RS07475 1577282..1577683 - 402 WP_003898861.1 type II toxin-antitoxin system VapC family toxin Toxin
JN141_RS07480 1577680..1577937 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
JN141_RS07485 1578020..1578979 - 960 WP_003407276.1 alpha/beta hydrolase -
JN141_RS07490 1579004..1579966 - 963 WP_003407279.1 alpha/beta hydrolase -
JN141_RS07495 1579965..1580702 + 738 WP_031647000.1 lysoplasmalogenase -
JN141_RS07500 1580783..1582750 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14952.43 Da        Isoelectric Point: 11.4656

>T189486 WP_003898861.1 NZ_CP069072:c1577683-1577282 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQDLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T189486 NZ_CP090782:2029813-2030097 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGACGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCACGGTCGCGCACTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT189486 WP_003407272.1 NZ_CP069072:c1577937-1577680 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT189486 NZ_CP090782:2029516-2029809 [Burkholderia multivorans]
ATGGTTACTTTGCTGCGATCCACGGAGTTCAACGATTGGCTTGCCAACCTGCGCGATCAGCGCGGCAAGGCCCGAATCGC
AGCCCGCCTGATCAGCGCGCAACTCGGCAACTTCGGCGAGTACCGTGTGCTCGACGAAGGGGTCTGCGAACTGAAGATCG
ATTTCGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGTGGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCA
ACGCAACGGAAGGACATCAAGCCCGTGCAGATGGCGCGCGAACTGAAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045ISB0

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References