Detailed information of TA system TA189482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1242647..1243215
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JN141_RS05965
• Protein ID: WP_003405865.1
• Coordinates: 1242841..1243215 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN141_RS05960
• Protein ID: WP_003405863.1
• Coordinates: 1242647..1242844 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS05940	1238688..1239326	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN141_RS05945	1239416..1240423	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN141_RS05950	1240440..1241423	-	984	WP_003898731.1	hypothetical protein	-
JN141_RS05955	1241486..1242559	+	1074	WP_031578409.1	redox-regulated ATPase YchF	-
JN141_RS05960	1242647..1242844	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN141_RS05965	1242841..1243215	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN141_RS05970	1243418..1244116	+	699	WP_003898733.1	hypothetical protein	-
JN141_RS05975	1244234..1244419	+	186	WP_003901093.1	hypothetical protein	-
JN141_RS05980	1244346..1244621	-	276	WP_003405867.1	hypothetical protein	-
JN141_RS05985	1244864..1245187	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN141_RS05990	1245202..1245900	-	699	WP_031646953.1	hypothetical protein	-
JN141_RS05995	1245934..1246143	+	210	WP_003911400.1	hypothetical protein	-
JN141_RS06000	1246095..1246865	-	771	Protein_1180	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T189482 WP_003405865.1 NZ_CP069072:1242841-1243215 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189482 NZ_CP090781:c1567217-1566798 [Burkholderia multivorans]
ATGATCCTTGTCGATACGAACGTCATTTTCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
CCAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGAC
GCCGGCGGGACTGGCTGGAGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGGGCAAAGCCTATGCGCGCATTCGTGCGCACGCCCGTGCCGTCGGCAACGCGATCGCGCCGGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCGTCATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189482 WP_003405863.1 NZ_CP069072:1242647-1242844 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189482 NZ_CP090781:c1567387-1567214 [Burkholderia multivorans]
CTGCACGGGCGCAGTACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTGGG
AACCTTGCTGGCGGAAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATCCAGCGTGACAAGACGCGAACCGACCCGA
TGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07