Detailed information of TA system TA189479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1027544..1028517
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: JN141_RS04890
• Protein ID: WP_003404756.1
• Coordinates: 1028017..1028517 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: O05910
• Locus tag: JN141_RS04885
• Protein ID: WP_003898647.1
• Coordinates: 1027544..1028020 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS04865	1023391..1024662	-	1272	WP_003404747.1	PPE family protein	-
JN141_RS04870	1024677..1024976	-	300	WP_003404748.1	PE family protein	-
JN141_RS04875	1025420..1027201	+	1782	WP_003404749.1	BCCT family transporter	-
JN141_RS04880	1027201..1027455	+	255	WP_003898646.1	hypothetical protein	-
JN141_RS04885	1027544..1028020	+	477	WP_003898647.1	DUF1778 domain-containing protein	Antitoxin
JN141_RS04890	1028017..1028517	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
JN141_RS04900	1028830..1030149	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
JN141_RS04905	1030437..1031018	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
JN141_RS04910	1031018..1032670	+	1653	WP_003404761.1	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T189479 WP_003404756.1 NZ_CP069072:1028017-1028517 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T189479 NZ_CP090781:996544-997119 [Burkholderia multivorans]
ATGACGCATTCGCCATTTACGGCGATCTACGATGCCTGTGTGCTTTATCCCGCGCCGCTTCGCGATTTCCTGATGCGGCT
GGCGTTGAGTGGATGTTTCCGGGCGCGGTGGAGCGCACATATTACCGACGAGTGGAAGCGCAATCTGTTGGACAACCGTC
CGGATTTGACAGCGGAACAGCTTGATCGAACCTCAAGTTTGATGGATCGAGCGATACCGGATGCCGTCGTGACCGGTTAT
GAATCGTTGATTGCCGGGCTTGTTCTCCCGGATGCCGACGATCGTCACGTGCTTGCCGCTGCAATCCGTTGCAACGCCAG
CGTGATCGTGACGTTCAATCGAAAGGACTTTCCGGAAGAGGCGTTGTTGCCGTACGGCATCGAGGCCCAGCATCCAGACG
AGTTCGTCGATGATCTTTTCGACCTCGAACCCGCGGTGGTCGTTGCGGCCGCGCGACGACAGCGAGAGGCGCTTAAACAA
CCACCGTTGACTGCAGACCGGTACCTCGAGATCTTGCGACGACAAGGCATGGTGCAGACGTGTCAGTCGCTCGACGCCTA
TCGAGCACTTCTTTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17387.98 Da
• Isoelectric Point: 11.2467

>AT189479 WP_003898647.1 NZ_CP069072:1027544-1028020 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT189479 NZ_CP090781:996080-996547 [Burkholderia multivorans]
ATGCATCACGCCACGACACCCGACATGATCTTGCCCGTCGAGCGCGAGATGCAGGCGGCCGTCGAGGGGCAACGCATGCT
GGCGGCTTATCTCGCAACACGGACCGATACGCAGCGCATTCAGATTTTTGATGACGAGAACCGAGCCCATCAGGTCGAGC
TGCCGACGTCCGCACTTCGTCTGCTGGTGGATATTCTCGCGGAATTGGCGGATGGCAACGCTGTCAAGGTTGTGCCGGTT
CATGCGGAATTGACGACGCAGGAAGCGGCAGATCTGCTCAATGTCTCCCGCCCGCATCTGATCAAGCTGCTTGAGTCGAA
TGCATTGCCCTATCACCGGACGGGCAAGCATCGGCGCGTGCGGTTTTCGGATCTGATGCGCTACAAGGCTGAGCGCGACC
AAGAGAGTGCTGACGCAATGGAAGAACTGAGCAAGCAGGCACAGGATCTGCGGATGGGCTACGAATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2

Antitoxin

• AlphaFold DB: A5U0V8