Detailed information of TA system TA189478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1018014..1018633
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN141_RS04835
• Protein ID: WP_003404726.1
• Coordinates: 1018199..1018633 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: JN141_RS04830
• Protein ID: WP_003898641.1
• Coordinates: 1018014..1018193 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS04815	1013487..1015067	+	1581	WP_160522503.1	serine hydrolase	-
JN141_RS04820	1015064..1017457	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
JN141_RS04825	1017730..1017888	+	159	WP_003404720.1	hypothetical protein	-
JN141_RS04830	1018014..1018193	+	180	WP_003898641.1	antitoxin	Antitoxin
JN141_RS04835	1018199..1018633	+	435	WP_003404726.1	SRPBCC family protein	Toxin
JN141_RS04840	1018731..1019504	+	774	WP_003404735.1	VOC family protein	-
JN141_RS04845	1019569..1020018	+	450	WP_003404738.1	hypothetical protein	-
JN141_RS04850	1020153..1020383	-	231	WP_003898642.1	hypothetical protein	-
JN141_RS04855	1020550..1022058	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
JN141_RS04860	1022060..1023298	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T189478 WP_003404726.1 NZ_CP069072:1018199-1018633 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T189478 NZ_CP090781:c405725-405408 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT189478 WP_003898641.1 NZ_CP069072:1018014-1018193 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT189478 NZ_CP090781:c405404-405105 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
TTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTCAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5