Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 757878..758418 Replicon chromosome
Accession NZ_CP069072
Organism Mycobacterium tuberculosis strain N1216

Toxin (Protein)


Gene name mazF Uniprot ID P9WII0
Locus tag JN141_RS03500 Protein ID WP_003403376.1
Coordinates 757878..758186 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag JN141_RS03505 Protein ID WP_003403381.1
Coordinates 758173..758418 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN141_RS03475 753193..754698 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
JN141_RS03480 754779..755789 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
JN141_RS03485 756177..756560 - 384 WP_003403365.1 ribonuclease VapC6 -
JN141_RS03490 756655..756810 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
JN141_RS03495 756886..757602 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
JN141_RS03500 757878..758186 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JN141_RS03505 758173..758418 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
JN141_RS03510 758528..758965 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
JN141_RS03515 758962..759216 - 255 WP_003911263.1 antitoxin VapB7 -
JN141_RS03520 759330..761692 + 2363 Protein_693 arylsulfatase AtsD -
JN141_RS03525 761755..762081 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN141_RS03530 761993..762331 + 339 WP_003403405.1 PIN domain-containing protein -
JN141_RS03535 762328..762501 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-101)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11320.10 Da        Isoelectric Point: 11.6554

>T189474 WP_003403376.1 NZ_CP069072:c758186-757878 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Download         Length: 309 bp

>T189474 NZ_CP090779:c1369186-1368902 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGACGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCACGGTCGCGCACTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT189474 WP_003403381.1 NZ_CP069072:c758418-758173 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT189474 NZ_CP090779:c1369483-1369190 [Burkholderia multivorans]
ATGGTTACTTTGCTGCGATCCACGGAGTTCAACGATTGGCTTGCCAACCTGCGCGATCAGCGCGGCAAGGCCCGAATCGC
AGCCCGCCTGATCAGCGCGCAACTCGGCAACTTCGGCGAGTACCGTGTGCTCGACGAAGGGGTCTGCGAACTGAAGATCG
ATTTCGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGTGGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCA
ACGCAACGGAAGGACATCAAGCCCGTGCAGATGGCGCGCGAACTGAAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSE5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References