Detailed information of TA system TA189470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 713442..714067
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQA0
• Locus tag: JN141_RS03265
• Protein ID: WP_003403218.1
• Coordinates: 713666..714067 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ36
• Locus tag: JN141_RS03260
• Protein ID: WP_003403213.1
• Coordinates: 713442..713669 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS03235	708621..708842	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
JN141_RS03240	708984..709589	+	606	WP_003898526.1	hypothetical protein	-
JN141_RS03245	709608..712175	-	2568	WP_003900188.1	SEC-C metal-binding domain-containing protein	-
JN141_RS03250	712259..713008	+	750	WP_003898528.1	hypothetical protein	-
JN141_RS03255	713005..713247	+	243	WP_003403210.1	hypothetical protein	-
JN141_RS03260	713442..713669	+	228	WP_003403213.1	antitoxin VapB29	Antitoxin
JN141_RS03265	713666..714067	+	402	WP_003403218.1	type II toxin-antitoxin system ribonuclease VapC29	Toxin
JN141_RS03270	714196..714279	+	84	Protein_646	galactose-1-phosphate uridylyltransferase	-
JN141_RS03275	714298..715379	+	1082	Protein_647	galactose-1-phosphate uridylyltransferase	-
JN141_RS03280	715376..716467	+	1092	WP_003906403.1	galactokinase	-
JN141_RS03285	716862..717926	+	1065	WP_003898532.1	zinc ribbon domain-containing protein	-
JN141_RS03290	718030..718977	+	948	WP_003403232.1	DUF732 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 13937.76 Da
• Isoelectric Point: 5.7441

>T189470 WP_003403218.1 NZ_CP069072:713666-714067 [Mycobacterium tuberculosis]
VTVLLDANVLIALVVAEHVHHDAAADWLMASDTGFATCPMTQGSLVRFLVRSGQSAAAARDVVSAVQCTSRHEFWPDALS
FAGVEVAGVVGHRQVTDAYLAQLARSHDGQLATLDSGLAHLHGDVAVLIPTTT

Nucleotide

• Length: 402 bp

>T189470 NZ_CP090778:c1362712-1362137 [Burkholderia multivorans]
ATGACGCATTCGCCATTTACGGCGATCTACGATGCCTGTGTGCTTTATCCCGCGCCGCTTCGCGATTTCCTGATGCGGCT
GGCGTTGAGTGGATGTTTCCGGGCGCGGTGGAGCGCACATATTACCGACGAGTGGAAGCGCAATCTGTTGGACAACCGTC
CGGATTTGACAGCGGAACAGCTTGATCGAACCTCAAGTTTGATGGATCGAGCGATACCGGATGCCGTCGTGACCGGTTAT
GAATCGTTGATTGCCGGGCTTGTTCTCCCGGATGCCGACGATCGTCACGTGCTTGCCGCTGCAATCCGTTGCAACGCCAG
CGTGATCGTGACGTTCAATCGAAAGGACTTTCCGGAAGAGGCGTTGTTGCCGTACGGCATCGAGGCCCAGCATCCAGACG
AGTTCGTCGATGATCTTTTCGACCTCGAACCCGCGGTGGTCGTTGCGGCCGCGCGACGACAGCGAGAGGCGCTTAAACAA
CCACCGTTGACTGCAGACCGGTACCTCGAGATCTTGCGACGACAAGGCATGGTGCAGACGTGTCAGTCGCTCGACGCCTA
TCGAGCACTTCTTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8163.17 Da
• Isoelectric Point: 5.6307

>AT189470 WP_003403213.1 NZ_CP069072:713442-713669 [Mycobacterium tuberculosis]
VRTTIDLPQDLHKQALAIARDTHRTLSETVADLMRRGLAANRPTALSSDPRTGLPLVSVGTVVTSEDVRSLEDEQ

Nucleotide

• Length: 228 bp

>AT189470 NZ_CP090778:c1363176-1362709 [Burkholderia multivorans]
ATGCATCACGCCACGACACCCGACATGATCTTGCCCGTCGAGCGCGAGATGCAGGCGGCCGTCGAGGGGCAACGCATGCT
GGCGGCTTATCTCGCAACACGGACCGATACGCAGCGCATTCAGATTTTTGATGACGAGAACCGAGCCCATCAGGTCGAGC
TGCCGACGTCCGCACTTCGTCTGCTGGTGGATATTCTCGCGGAATTGGCGGATGGCAACGCTGTCAAGGTTGTGCCGGTT
CATGCGGAATTGACGACGCAGGAAGCGGCAGATCTGCTCAATGTCTCCCGCCCGCATCTGATCAAGCTGCTTGAGTCGAA
TGCATTGCCCTATCACCGGACGGGCAAGCATCGGCGCGTGCGGTTTTCGGATCTGATGCGCTACAAGGCTGAGCGCGACC
AAGAGAGTGCTGACGCAATGGAAGAACTGAGCAAGCAGGCACAGGATCTGCGGATGGGCTACGAATGA

Structures

Toxin

• AlphaFold DB: G0TQA0

Antitoxin

• AlphaFold DB: A0A7U4BSC9