Detailed information of TA system TA189462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 366175..366746
• Replicon: chromosome
• Accession: NZ_CP069072
• Organism: Mycobacterium tuberculosis strain N1216

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: JN141_RS01590
• Protein ID: WP_003401560.1
• Coordinates: 366175..366477 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: JN141_RS01595
• Protein ID: WP_003401563.1
• Coordinates: 366525..366746 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN141_RS01570	361644..362447	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
JN141_RS01575	362457..363854	-	1398	WP_003401544.1	sulfatase	-
JN141_RS01580	364033..365808	+	1776	WP_010886074.1	PE family protein	-
JN141_RS01585	365951..366178	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
JN141_RS01590	366175..366477	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
JN141_RS01595	366525..366746	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
JN141_RS01600	366743..367168	+	426	WP_003401566.1	PIN domain nuclease	-
JN141_RS01605	367304..367936	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
JN141_RS01610	367933..368841	+	909	WP_003900117.1	protochlorophyllide reductase	-
JN141_RS01615	368849..369439	-	591	WP_229298012.1	hypothetical protein	-
JN141_RS01620	369432..369482	-	51	Protein_320	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T189462 WP_003401560.1 NZ_CP069072:366175-366477 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T189462 NZ_CP090776:c759425-759060 [Burkholderia multivorans]
ATGGTAAAAGCTCTCTTCGACACCAACATCCTGATCGACTATCTGAGCGGCGTGGAGTCGGCCCGCCTGGAGCTCGGCCG
CTACGACCACCGTGCGATCAGCACCATTACGTGGATGGAAGTCCTGGTCGGTACGACCGCAGATGACGAAGGCCCGATTC
GCGCGTGGCTCTCGTCGTTCGACGTGATCCCGCTCGACAGTACGGTTGCCGACCGCGCCGTGACCATTCGCAAGGAACGG
CGCATTCGTCTGCCCGACGCGATCGTCTGGGCATCAGCGCAGGTGAACGGACTCCTGCTCGTGTCGCGCAACACGAAAGA
CTTTCCCTCGACGGAACCCGGCATCCGGGTGCCTTACCAGATCTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT189462 WP_003401563.1 NZ_CP069072:366525-366746 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT189462 NZ_CP090776:c759637-759419 [Burkholderia multivorans]
ATGAGCCGAGTCTTGGTGGATCTGTCGGACGGCCAGCTCGACGAGCTGGCCGTCATCGTCGAGACTGAGCATCGTCCTCG
CGCCGCGATCATTCGCGATGCGATTGACGCCTACATCGCCCTACACAAGCGCCCGCTCGCCGACGATGTCTTCGGCCTCT
GGAAAGACCGCGCCGTCGACGGGCTCGCTTATCAGGAAAAACTGCGCTCGGAATGGTAA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48