Detailed information of TA system TA189457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3763053..3763582
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: JN142_RS17725
• Protein ID: WP_003417760.1
• Coordinates: 3763325..3763582 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: JN142_RS17720
• Protein ID: WP_003417757.1
• Coordinates: 3763053..3763328 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS17685	3759626..3760315	-	690	WP_003917774.1	BBE domain-containing protein	-
JN142_RS17690	3760502..3760873	-	372	WP_003417739.1	FAD-binding protein	-
JN142_RS17695	3760771..3760989	-	219	WP_157132559.1	hypothetical protein	-
JN142_RS17700	3761016..3761276	-	261	WP_003417741.1	hypothetical protein	-
JN142_RS17705	3761391..3761780	+	390	WP_003417745.1	DUF732 domain-containing protein	-
JN142_RS17710	3761794..3762087	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
JN142_RS17715	3762084..3762929	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
JN142_RS17720	3763053..3763328	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
JN142_RS17725	3763325..3763582	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
JN142_RS17730	3763624..3764814	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
JN142_RS17735	3764931..3765299	+	369	WP_003417765.1	FHA domain-containing protein	-
JN142_RS17740	3765296..3765847	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
JN142_RS17745	3765854..3766435	-	582	WP_003417769.1	ATP/GTP-binding protein	-
JN142_RS17750	3766416..3766784	-	369	WP_003417772.1	DUF742 domain-containing protein	-
JN142_RS17755	3766762..3767154	-	393	WP_003417776.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T189457 WP_003417760.1 NZ_CP069071:3763325-3763582 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T189457 NZ_CP090774:c1090863-1090486 [Burkholderia multivorans]
ATGGGTATAAATTCATCGATGGTTCCGTACACCAAACCGCTCTACTGGCTCGGCTCGGCACGCAAGGACTTGAAAGCGAT
GCCCGAAGCGGTACAGGACACGTTCGGCTATGCGCTGTATCTGGCCCAGACGGGAGGAAAGCACGATCAGGCCAAGCCGT
TGAGGGGTTTCGGATCGGCCGGCGTACTCGAGGTCGTGGAGTCGGAGCACAGCGGCACGTACCGGGCGGTCTATACGGTG
AAACTCGGTGGCGCCGTCTATGTGCTGCACTGTTTCCAGAAGAAATCGTGCCGCGGTATCGCTACGCCGAAGCCGGACAT
CGAACTGGTTCGCGCGCGCCTGAGCGCCGCCGAAGCACACGCAAATGGAGAAAAGTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT189457 WP_003417757.1 NZ_CP069071:3763053-3763328 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT189457 NZ_CP090774:c1090486-1090163 [Burkholderia multivorans]
ATGATCGAAATCGAACGGGGCAGCGGGAACGTCTACGCGGATCTTGGCACGCCCGACGCCGAAGAAATGCGCGTCAAGGC
TCAACTCGCCGCCAAGATCGCGGAGATCATCAAGACTCGCAAATGGACGCAGCAGCACGCAGCACGATTGCTCGGCATGA
CGCAACCGAAGCTATCGAACATGCTGCGCGGGCAGTTTCGCGGCGTAAGCGAAGCGAAGATGCTCGACTGTCTCGCCAAG
CTCGGGCGCGATGTACAGATCGTCGTAGGACCGGACCGTCATTCGGAAACCGAAGGACACATCGAGGTCGTGTTCGCTGC
CTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94