Detailed information of TA system TA189450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3102849..3103419
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: JN142_RS14775
• Protein ID: WP_003414166.1
• Coordinates: 3102849..3103205 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: JN142_RS14780
• Protein ID: WP_003901465.1
• Coordinates: 3103189..3103419 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS14755	3098301..3099989	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
JN142_RS14760	3099993..3100319	-	327	WP_003414157.1	hypothetical protein	-
JN142_RS14765	3100492..3101079	+	588	WP_003914429.1	DUF3558 family protein	-
JN142_RS14770	3101098..3102747	+	1650	WP_070171102.1	CocE/NonD family hydrolase	-
JN142_RS14775	3102849..3103205	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
JN142_RS14780	3103189..3103419	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
JN142_RS14785	3103462..3104505	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
JN142_RS14790	3104504..3104971	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
JN142_RS14795	3105147..3105401	-	255	WP_003917684.1	hypothetical protein	-
JN142_RS14800	3105549..3105953	+	405	WP_009938577.1	hypothetical protein	-
JN142_RS14805	3105950..3106141	+	192	WP_003414184.1	hypothetical protein	-
JN142_RS14810	3106358..3106618	+	261	Protein_2924	transposase	-
JN142_RS14815	3107728..3107985	+	258	WP_003899489.1	hypothetical protein	-
JN142_RS14820	3108090..3108401	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T189450 WP_003414166.1 NZ_CP069071:c3103205-3102849 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T189450 NZ_CP090771:c3493876-3493475 [Burkholderia multivorans]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTATCTGATGAAGAACCAGCCGGAGCAGGTCGCGCGGCGTTTCGC
GCAGTGCTACACGGGGGACGTCGTCATGTCGGCGATCACCTATGCGGAGCTCGAGTACGGCGTAACGCTATGCGCCAATC
CGGCCCGCGAACGCCGCAATCTCGCCGCGCTGATCGAGGATATCCCGGTGGCGCCGTTCGACGTTGCGGCTGCGCAGGCA
TACGGCCCCATTCGAGAGGCGACGCGCGAACGCAAGAAGGATCATCTCGACAAGCTGATCGGCGCGCACGCCGTATCGCT
CGACGTCGTGCTGGTGACCAACAACGAGCGTGATTTCGCGAGTTATCCGGGCTTGAGGCTGGAGAACTGGCTGATCGGCT
AG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT189450 WP_003901465.1 NZ_CP069071:c3103419-3103189 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT189450 NZ_CP090771:c3494112-3493876 [Burkholderia multivorans]
ATGCATACCACCCGGGTCTTCCGGAATGGCAACTCGCAGGCGGTGCGGATTCCTGCGGATCTTGCTTATGAGCGCAGCGA
TATCGAACTCGAAATCGAGCGTGTCGGTGACGAAATTCGCATCCGGCCTGCGCGGCGGCCGCTGACCGGCGTGCTCGGCA
AGTTCGCGAAATTCGGGCCCGATTTCATGGCCGAGGGGCGCGGCGATCAGGAACAGGCCGAGCGCGAGGGTTGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3