Detailed information of TA system TA189448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3062852..3063531
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JN142_RS14545
• Protein ID: WP_003414059.1
• Coordinates: 3062852..3063268 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN142_RS14550
• Protein ID: WP_003414061.1
• Coordinates: 3063265..3063531 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS14520	3058904..3059806	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN142_RS14525	3059875..3060627	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN142_RS14535	3060871..3061146	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN142_RS14540	3061143..3062765	-	1623	WP_003906897.1	class I SAM-dependent DNA methyltransferase	-
JN142_RS14545	3062852..3063268	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JN142_RS14550	3063265..3063531	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN142_RS14555	3063557..3063952	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JN142_RS14560	3063949..3064218	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN142_RS14565	3064228..3065322	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN142_RS14570	3065319..3065738	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN142_RS14575	3065737..3065811	+	75	Protein_2877	hypothetical protein	-
JN142_RS14580	3065812..3066291	-	480	WP_003414073.1	dihydrofolate reductase	-
JN142_RS14585	3066362..3067162	-	801	WP_009939104.1	thymidylate synthase	-
JN142_RS14590	3067318..3068055	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T189448 WP_003414059.1 NZ_CP069071:c3063268-3062852 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T189448 NZ_CP090771:c1088414-1088037 [Burkholderia multivorans]
ATGGGTATAAATTCATCGATGGTTCCGTACACCAAACCGCTCTACTGGCTCGGCTCGGCACGCAAGGACTTGAAAGCGAT
GCCCGAAGCGGTACAGGACACGTTCGGCTATGCGCTGTATCTGGCCCAGACGGGAGGAAAGCACGATCAGGCCAAGCCGT
TGAGGGGTTTCGGATCGGCCGGCGTACTCGAGGTCGTGGAGTCGGAGCACAGCGGCACGTACCGGGCGGTCTATACGGTG
AAACTCGGTGGCGCCGTCTATGTGCTGCACTGTTTCCAGAAGAAATCGTGCCGCGGTATCGCTACGCCGAAGCCGGACAT
CGAACTGGTTCGCGCGCGCCTGAGCGCCGCCGAAGCACACGCAAATGGAGAAAAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189448 WP_003414061.1 NZ_CP069071:c3063531-3063265 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189448 NZ_CP090771:c1088037-1087714 [Burkholderia multivorans]
ATGATCGAAATCGAACGGGGCAGCGGGAACGTCTACGCGGATCTTGGCACGCCCGACGCCGAAGAAATGCGCGTCAAGGC
TCAACTCGCCGCCAAGATCGCGGAGATCATCAAGACTCGCAAATGGACGCAGCAGCACGCAGCACGATTGCTCGGCATGA
CGCAACCGAAGCTATCGAACATGCTGCGCGGGCAGTTTCGCGGCGTAAGCGAAGCGAAGATGCTCGACTGTCTCGCCAAG
CTCGGGCGCGATGTACAGATCGTCGTAGGACCGGACCGTCATTCGGAAACCGAAGGACACATCGAGGTCGTGTTCGCTGC
CTGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64