Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2863618..2864255 Replicon chromosome
Accession NZ_CP069071
Organism Mycobacterium tuberculosis strain N0136

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN142_RS13455 Protein ID WP_003413180.1
Coordinates 2863618..2864013 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN142_RS13460 Protein ID WP_003413183.1
Coordinates 2864010..2864255 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN142_RS13415 2859021..2860232 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
JN142_RS13420 2860359..2861018 + 660 WP_003900846.1 LppA family lipoprotein -
JN142_RS13425 2861015..2861677 + 663 WP_003900848.1 LppA family lipoprotein -
JN142_RS13430 2861674..2861952 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN142_RS13435 2862045..2862458 + 414 WP_003413164.1 PIN domain nuclease -
JN142_RS13440 2862497..2862754 + 258 WP_003413167.1 CopG family transcriptional regulator -
JN142_RS13445 2862751..2863128 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN142_RS13450 2863144..2863518 - 375 WP_003413177.1 hypothetical protein -
JN142_RS13455 2863618..2864013 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN142_RS13460 2864010..2864255 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN142_RS13465 2864666..2865085 - 420 WP_003413190.1 A24 family peptidase -
JN142_RS13470 2865097..2865906 - 810 WP_003413193.1 shikimate dehydrogenase -
JN142_RS13475 2865903..2867156 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN142_RS13480 2867149..2867661 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189445 WP_003413180.1 NZ_CP069071:c2864013-2863618 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189445 NZ_CP090769:2452876-2453175 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
TTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATTCTGAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189445 WP_003413183.1 NZ_CP069071:c2864255-2864010 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189445 NZ_CP090769:2452555-2452872 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References