Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2862497..2863128 Replicon chromosome
Accession NZ_CP069071
Organism Mycobacterium tuberculosis strain N0136

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag JN142_RS13445 Protein ID WP_003413174.1
Coordinates 2862751..2863128 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag JN142_RS13440 Protein ID WP_003413167.1
Coordinates 2862497..2862754 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN142_RS13410 2858411..2858725 + 315 WP_009937839.1 hypothetical protein -
JN142_RS13415 2859021..2860232 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
JN142_RS13420 2860359..2861018 + 660 WP_003900846.1 LppA family lipoprotein -
JN142_RS13425 2861015..2861677 + 663 WP_003900848.1 LppA family lipoprotein -
JN142_RS13430 2861674..2861952 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN142_RS13435 2862045..2862458 + 414 WP_003413164.1 PIN domain nuclease -
JN142_RS13440 2862497..2862754 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
JN142_RS13445 2862751..2863128 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
JN142_RS13450 2863144..2863518 - 375 WP_003413177.1 hypothetical protein -
JN142_RS13455 2863618..2864013 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
JN142_RS13460 2864010..2864255 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
JN142_RS13465 2864666..2865085 - 420 WP_003413190.1 A24 family peptidase -
JN142_RS13470 2865097..2865906 - 810 WP_003413193.1 shikimate dehydrogenase -
JN142_RS13475 2865903..2867156 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN142_RS13480 2867149..2867661 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T189444 WP_003413174.1 NZ_CP069071:2862751-2863128 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T189444 NZ_CP090769:2452555-2452872 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT189444 WP_003413167.1 NZ_CP069071:2862497-2862754 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT189444 NZ_CP090769:2452876-2453175 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
TTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATTCTGAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References