Detailed information of TA system TA189443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2862045..2862754
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ26
• Locus tag: JN142_RS13435
• Protein ID: WP_003413164.1
• Coordinates: 2862045..2862458 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: JN142_RS13440
• Protein ID: WP_003413167.1
• Coordinates: 2862497..2862754 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS13405	2857098..2858303	-	1206	WP_003413027.1	chorismate synthase	-
JN142_RS13410	2858411..2858725	+	315	WP_009937839.1	hypothetical protein	-
JN142_RS13415	2859021..2860232	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
JN142_RS13420	2860359..2861018	+	660	WP_003900846.1	LppA family lipoprotein	-
JN142_RS13425	2861015..2861677	+	663	WP_003900848.1	LppA family lipoprotein	-
JN142_RS13430	2861674..2861952	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
JN142_RS13435	2862045..2862458	+	414	WP_003413164.1	PIN domain nuclease	Toxin
JN142_RS13440	2862497..2862754	+	258	WP_003413167.1	CopG family transcriptional regulator	Antitoxin
JN142_RS13445	2862751..2863128	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
JN142_RS13450	2863144..2863518	-	375	WP_003413177.1	hypothetical protein	-
JN142_RS13455	2863618..2864013	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JN142_RS13460	2864010..2864255	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JN142_RS13465	2864666..2865085	-	420	WP_003413190.1	A24 family peptidase	-
JN142_RS13470	2865097..2865906	-	810	WP_003413193.1	shikimate dehydrogenase	-
JN142_RS13475	2865903..2867156	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
JN142_RS13480	2867149..2867661	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15045.00 Da
• Isoelectric Point: 5.4673

>T189443 WP_003413164.1 NZ_CP069071:2862045-2862458 [Mycobacterium tuberculosis]
MVFCVDTSAWHHAARPEVARRWLAALSADQIGICDHVRLEILYSANSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTIVWHYDENYDRVAAITGQPTEWIVPRGTL

Nucleotide

• Length: 414 bp

>T189443 NZ_CP090769:1509969-1510334 [Burkholderia multivorans]
ATGGTAAAAGCCCTCTTCGACACCAACATCCTGATCGACTATCTGAGCGGCGTGGAGTCGGCCCGCCTGGAGCTCGGCCG
CTACGACCACCGTGCAATCAGCACCATTACGTGGATGGAAGTCCTGGTCGGTACGACCGCAGATGACGAAGGCCCGATTC
GCGCGTGGCTCTCGTCGTTCGACGTGATCCCGCTCGACATTACGGTTGCCGACCGCGCCGTGACCATTCGCAAGGAACGG
CGCATTCGTCTGCCCGACGCGATCGTCTGGGCATCAGCGCAGGTGAACGGACGCCTGCTCGTGTCGCGCAACACGAAAGA
CTTTCCCTCGACGGAACCCGGCATCCGGGTGCCTTACCAGATCTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT189443 WP_003413167.1 NZ_CP069071:2862497-2862754 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT189443 NZ_CP090769:1509757-1509975 [Burkholderia multivorans]
ATGAGCCGAGTCTTGGTGGATCTGTCGGACGGCCAGCTCGACGAGCTGGCCGTCATCGTCGAGACTGAGCATCGTCCTCG
CGCCGCGATCATTCGCGATGCGATTGACGCCTACATCGCCCTACACAAGCGCCCGCTCGCCGACGATGTCTTCGGCCTCT
GGAAAGACCGCGCCGTCGACGGGCTCGCTTATCAGGAAAAACTGCGCTCGGAATGGTAA

Structures

Toxin

• AlphaFold DB: G0TQ26

Antitoxin

• AlphaFold DB: A0A829C9W5