Detailed information of TA system TA189442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2848158..2848798
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JN142_RS13350
• Protein ID: WP_003412970.1
• Coordinates: 2848158..2848577 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JN142_RS13355
• Protein ID: WP_003412975.1
• Coordinates: 2848574..2848798 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS13320	2843743..2844465	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JN142_RS13325	2844982..2845209	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
JN142_RS13330	2845206..2845607	+	402	WP_003412963.1	PIN domain-containing protein	-
JN142_RS13335	2845642..2846562	-	921	WP_003412965.1	restriction endonuclease	-
JN142_RS13340	2846903..2847109	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN142_RS13345	2847207..2848157	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
JN142_RS13350	2848158..2848577	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN142_RS13355	2848574..2848798	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JN142_RS13360	2848829..2851672	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN142_RS13365	2851744..2852145	-	402	WP_003412981.1	hypothetical protein	-
JN142_RS13370	2852145..2852615	-	471	WP_003899364.1	transcription antitermination factor NusB	-
JN142_RS13375	2852618..2853181	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189442 WP_003412970.1 NZ_CP069071:c2848577-2848158 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189442 NZ_CP090769:918918-919814 [Burkholderia multivorans]
ATGAGTGTCGTCGACAAACGAGAACGCGACCGCACCGCGGCGTTCGCCACGCTGCTCGAGCGGTTTCCCGAGCAGCGCTG
CGCCGCCGGCGCGGCGGGCACGATCGGTTATCGCGAGGCCGGTGCGCAGCAGGCTGGCGCCGCGCTGCCCGTCGTGCTGC
TGCACGGGATCGGCTCGGGCGCCGCGTCGTGGGTGCGCCAGCTCGACGCGCTCGGCGCTTCGCGCCGCGTGCTCGCGTGG
GACGCGCCCGGCTACGGCGCATCGACGCCGGTGCACGGCGCATCGCCGTCGGCCGTCGATTATGCGGCGTCGCTCGACGC
GTGGCTCGACGCGCTCGGCATCGCGCGCTGCGTGCTCGTCGGGCATTCGCTCGGCGCGATCGTCGCGGGCGGCATGGTGC
GCGTGAAGCCCGAGCGGATCGCGGGGCTGCTGCTGATTTCGCCGGCCGGCGGATACGGCAGCGCGCCGGCCGAGACGCGC
GCGTCGCGCCGCGACGCGCGGCTCGCGATGCTCGCGGAACTCGGGCCTGCCGGGCTCGCCGCGCAGCGCAGCGGCAACAT
GCTGTCGGGCTTCGCGACCGAGGACGCGCGCGCATGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCGC
AGGCCACGCATCTGCTCGCCAATGCCGATCTCGCGGCCGATCTCGCCGGGTTCCGCGGCCGCACGGCGGTGGCCGTGGGC
GCGAACGACGCGATTACGCCGCCCGCGGCGTGCGAACGCATCGCGGCGGCCGCCGGCGTCGGACTGCAGGTGATTCCGCA
GGCCGGCCACGCGGGCTACGTCGAGGCGCCCGACGTCTATTCGGCCTTGATCGACGCGTTCTGCCGGCAGTGCGACATCG
AGCGGAGGGCGGCATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT189442 WP_003412975.1 NZ_CP069071:c2848798-2848574 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189442 NZ_CP090769:919811-920665 [Burkholderia multivorans]
ATGAGCGAACCGTTGCAGAGCGCGGCCGAACCTGCGAAGGCCGAAGCGAGCTACGTCGTGCCCGGCCTCGAGCGCGGGCT
GCGGATTCTCGCCGAATTCTCGCCGCGCGAACCGGTACTCGGCGCGCCGGAGCTGTCGAAGCGGCTCGGCATTCCGCGCA
CGACCGTATTCCGTCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAGCGCGCCGACAAGGACCGCAACTACAAGCTC
GGCATCGCGGTGCTGCGGCTCGGCTTCGAATATCTGAGCTCGCTCGAGCTGACCGATCTCGGCCTGCCCGTGATCGAAAG
CCTGCGCGATGCGACCGGCTTCACGACGCACATCGTGATCCGCGACGGCCGCGACGTCGTGTTCGTCGCGAAGGCGCAGA
GCGAGGCGCCCGTGTTCAGTTCGATTCGCGTGAACGTCGGTACGCGGCTGCCCGCGCACGCGACGACGCACGGTCACGTG
CTGATGGGCGACCTGTCGCTGGACGAGCTGCGCGCGCTGTATCCGGAAGGCACGCTGCCGCGGATGACGAACGCGACGCC
GGACACGGTCGACGCGCTGTACACCATGATCCGCGAGGATGCGCTGCGCGGATACGGCATCAGCAATTCGTCGTTCGAGC
GCGGGATTTCGGTCGTCACCGCGCCGGTGCGCAACGACACGCAGAAGATCGTCGCCTGCATCACGGTGACGGTGCCGCGC
CCGGAGATCGACGCGACGCTGATCGCCGACGGGCTGATCGACAAGGTGCGGCGCGCGGCGGCCGAACTGTCGCGGCGGCT
CAATTACCGCTCGGACGACGAGCACACGTTTTTGAAGGCTTTGGGACTCCGATGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09