Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2317664..2318300 Replicon chromosome
Accession NZ_CP069071
Organism Mycobacterium tuberculosis strain N0136

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag JN142_RS10840 Protein ID WP_003410654.1
Coordinates 2317890..2318300 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag JN142_RS10835 Protein ID WP_003410651.1
Coordinates 2317664..2317897 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN142_RS10820 2313121..2313513 + 393 WP_229303073.1 metal ABC transporter permease -
JN142_RS10825 2313514..2313918 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
JN142_RS10830 2314002..2317586 - 3585 WP_003410648.1 cobaltochelatase subunit CobN -
JN142_RS10835 2317664..2317897 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
JN142_RS10840 2317890..2318300 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
JN142_RS10845 2318284..2319375 + 1092 WP_003900454.1 precorrin-3B synthase -
JN142_RS10850 2319385..2320011 + 627 WP_003410658.1 precorrin-8X methylmutase -
JN142_RS10855 2320008..2321534 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
JN142_RS10860 2321480..2322703 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T189438 WP_003410654.1 NZ_CP069071:2317890-2318300 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T189438 NZ_CP090766:1960166-1960450 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGCCGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCAAGGTCGCGCACTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT189438 WP_003410651.1 NZ_CP069071:2317664-2317897 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT189438 NZ_CP090766:1959992-1960162 [Burkholderia multivorans]
GTGCTCGACGAAGGGGTCTGCGAACTCAAGATCGACTTTGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGT
GGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCAACGCAACGGAAGGACGTCAAGCGCGCCGTGCAGATGGCGCGCGAAC
TGAAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References