Detailed information of TA system TA189430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2186474..2187177
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JN142_RS10170
• Protein ID: WP_003409778.1
• Coordinates: 2186474..2186803 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JN142_RS10175
• Protein ID: WP_003409780.1
• Coordinates: 2186800..2187177 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS10150	2182857..2183927	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
JN142_RS10155	2183924..2184439	+	516	WP_003409718.1	flavin reductase family protein	-
JN142_RS10160	2184436..2185497	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JN142_RS10165	2185494..2186264	+	771	WP_003409775.1	SDR family oxidoreductase	-
JN142_RS10170	2186474..2186803	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JN142_RS10175	2186800..2187177	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JN142_RS10180	2187174..2187764	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
JN142_RS10185	2187819..2189183	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
JN142_RS10190	2189338..2189790	-	453	WP_003899095.1	lipoprotein	-
JN142_RS10195	2189854..2190255	+	402	WP_003409869.1	hypothetical protein	-
JN142_RS10200	2190248..2190430	-	183	WP_003409870.1	hypothetical protein	-
JN142_RS10205	2190544..2190894	-	351	WP_003899096.1	hypothetical protein	-
JN142_RS10210	2190905..2191807	-	903	WP_003899097.1	hypothetical protein	-
JN142_RS10215	2191828..2192019	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T189430 WP_003409778.1 NZ_CP069071:c2186803-2186474 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T189430 NZ_CP090765:772867-773322 [Burkholderia multivorans]
ATGATCCTTGTTGATACGAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
ACAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTGGCGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGAGCAAGGCCTATGCGCGCATTCGCGCGCACGCCCGTGCCGGCGGCAACGCGATCGCGCCCGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCGTCGCAGGGCGCGCCGGTGCGCCAGGGCATCGCGTCGAAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT189430 WP_003409780.1 NZ_CP069071:c2187177-2186800 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT189430 NZ_CP090765:772631-772870 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCACTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGTGTGAAGCTGGGAACCTTGCTGGCGG
AAATCGGACGGGAGGCAGGCGGTGTCGATTTCGACATTCAGCGTGACAAGACGCCAACCGATCCGATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8