Detailed information of TA system TA189427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1767496..1768109
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: JN142_RS08305
• Protein ID: WP_003407786.1
• Coordinates: 1767705..1768109 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: JN142_RS08300
• Protein ID: WP_009935474.1
• Coordinates: 1767496..1767699 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS08270	1762901..1763281	+	381	WP_003407768.1	fumarate reductase subunit C	-
JN142_RS08275	1763278..1763655	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
JN142_RS08280	1763723..1764331	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
JN142_RS08285	1764515..1765663	+	1149	Protein_1634	MMPL family transporter	-
JN142_RS08290	1765673..1766119	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
JN142_RS08295	1766154..1767443	+	1290	WP_003407781.1	threonine ammonia-lyase	-
JN142_RS08300	1767496..1767699	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN142_RS08305	1767705..1768109	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
JN142_RS08310	1768126..1769868	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
JN142_RS08315	1769861..1772158	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T189427 WP_003407786.1 NZ_CP069071:1767705-1768109 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T189427 NZ_CP090764:2353942-2354523 [Burkholderia cenocepacia]
ATGAATCCCGATACGATCCGCGCGCGCCTCGATTTCCTGCGCGACGCCGAACGCCTGAAAGACGTGCTGCGCAGCGGCCA
CACGTCGGCCGGGCGCGCGGAAAGCACGGCTGAGCATAGCTGGCGGCTGTGCCTGATGGCGCTCGTGTTCGCCGACGCGC
TGCCCGGCGTCGATACGCTGAAGCTGCTGAAACTGTGCGTCGTCCACGACCTCGGCGAGGCGCTGCACGGCGACATCCCC
GCGATCGAGCAGGCCGCGCACCCCGACAAGAGCGCGCACGAACGCGACGATCTGCTGACGCTGACCGCGTCGCTCGACCG
CGCGGCGCGCGACGAGATCGTCGCGCTGTGGGACGAATACGAGGCAGCCACCTCGCCGGAAGCCCGCGCGGCGAAAGCGC
TCGACAAGCTCGAAACGATCCTGCAGCACAACCAGGGCAACAATCCGCCCGATTTCGACTATGCGTTCAATCTCGGCTAC
GGCCGGCGCTATACCGACGCCGCGCCGCTGTTCAGCGCGATCCGCGAGATCGTCGATGTCGACACGCAGCGCAGGATCGA
CGCGGGTGGGCAGCGCGCGTAG

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT189427 WP_009935474.1 NZ_CP069071:1767496-1767699 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT189427 NZ_CP090764:2353297-2353863 [Burkholderia cenocepacia]
ATGCCCCACCCGCCGCTTCATCCATCCTCGCTCGGCCCGCAACTCAAGCGCTGGCGCGCGCTGCACCGCGTAAAGCAAAG
CCATGCAGCCGAACTGTTCCGCGTCGCGCAATCGACGATCTCCCGCTGGGAAGCCGGCCTTCAGCAGATGTCGCCCGCCG
AGCGCGCGACGGCCGAACGGCTGCTCGCCGCGCGCCTCGATTCCGCGGGCGACCACGCGCTCGCGCGGCTGATCGCCGGC
AGCACGGGCCGCATGCATCTCGTGTGCGACCTCACGCACCGCCTGCTGGCCAGCTCGCCGGCGCGCGCGGCCGAGTTCTC
GCGGCCGCTTTCGACGCTGCTCGGCGCGTCGCTGTGGCGCTATGCGACGCCGGAAATCGCCCGCATGGAAGCCGCGCTCG
ACCCGCTCGGCTGGTACGACCGCGCGGGACCGCCGAGCGTCGAGTTCGATACCGGCGCGAACGCGTCGCGCGTCGTGCCG
ATTCGCGGCAGCCTGTGCCGATGGACGCGGATGACGCTGTCGGACGGCTCGGCCGTGCGGCTCGTGGAAACGCTGGACGC
GCACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46