Detailed information of TA system TA189421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1240669..1241237
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JN142_RS05945
• Protein ID: WP_003405865.1
• Coordinates: 1240863..1241237 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN142_RS05940
• Protein ID: WP_003405863.1
• Coordinates: 1240669..1240866 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS05920	1236710..1237348	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN142_RS05925	1237438..1238445	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN142_RS05930	1238462..1239445	-	984	WP_003898731.1	hypothetical protein	-
JN142_RS05935	1239508..1240581	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JN142_RS05940	1240669..1240866	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN142_RS05945	1240863..1241237	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN142_RS05950	1241440..1242138	+	699	WP_003898733.1	hypothetical protein	-
JN142_RS05955	1242256..1242441	+	186	WP_003901093.1	hypothetical protein	-
JN142_RS05960	1242368..1242643	-	276	WP_003405867.1	hypothetical protein	-
JN142_RS05965	1242886..1243209	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN142_RS05970	1243224..1243922	-	699	WP_031646953.1	hypothetical protein	-
JN142_RS05975	1243956..1244165	+	210	WP_003911400.1	hypothetical protein	-
JN142_RS05980	1244117..1244887	-	771	Protein_1178	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T189421 WP_003405865.1 NZ_CP069071:1240863-1241237 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189421 NZ_CP090763:c3426463-3426062 [Burkholderia cenocepacia]
ATGCCGCGCTTCATGCTCGACACCAATATGTGTATCTATCTGATGAAAAATCAGCCTGAGCAGGTCGCGAGACGGTTTGC
GCGGTGCTACACGGGCGACGTCGTCATGTCGGCGATCACCTATGCGGAGCTCGAGTGCGGCCTGACCGCTTGTGTGAATC
CCGCGCGGGAGCGCCGCCATCTCGCCGCATTGATCGAGGATATTCCCGTCGCGCCTTTCGACGCGGCTGCCGCGCAAGCG
TATGGCCCGGTTCGCGAGGCCACCCGCGAGCGGAAGAAGGATCACCTCGACAAACTGATTGCCGCGCATGCGGTGTCGCT
CGATGTCGTCCTTGTCACCAACAACGAGCGCGATTTCGTCAGCTATCCGGGATTGCGACTGGAGAACTGGCTGAACGACT
AG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189421 WP_003405863.1 NZ_CP069071:1240669-1240866 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189421 NZ_CP090763:c3426699-3426463 [Burkholderia cenocepacia]
ATGCATACCACGAGGGTATTCAGGAACGGCAACTCGCAGGCCGTGCGAATTCCTGCGGACCTTGCCTATGAGCGCAGCGA
TATCGAACTGGAAATTGAGCGCGTCGGTGACGAAATCCGCATTCGGCCGATGCGGCGGCCTTTGACTCAGGTACTGGCGA
AGTTCGCGAAATTCGGGCCGGACTTCATGGCCGATGGGCGTGGCGACCACGAGCAGGCCGAACGCGAGGGTTTGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07