Detailed information of TA system TA189417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1014892..1015511
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN142_RS04810
• Protein ID: WP_003404726.1
• Coordinates: 1015077..1015511 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: JN142_RS04805
• Protein ID: WP_003898641.1
• Coordinates: 1014892..1015071 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS04790	1010365..1011945	+	1581	WP_003909313.1	serine hydrolase	-
JN142_RS04795	1011942..1014335	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
JN142_RS04800	1014608..1014766	+	159	WP_003404720.1	hypothetical protein	-
JN142_RS04805	1014892..1015071	+	180	WP_003898641.1	antitoxin	Antitoxin
JN142_RS04810	1015077..1015511	+	435	WP_003404726.1	SRPBCC family protein	Toxin
JN142_RS04815	1015609..1016382	+	774	WP_003404735.1	VOC family protein	-
JN142_RS04820	1016447..1016896	+	450	WP_003404738.1	hypothetical protein	-
JN142_RS04825	1017031..1017261	-	231	WP_003898642.1	hypothetical protein	-
JN142_RS04830	1017428..1018936	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
JN142_RS04835	1018938..1020176	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T189417 WP_003404726.1 NZ_CP069071:1015077-1015511 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T189417 NZ_CP090761:c1886914-1886507 [Burkholderia cenocepacia]
ATGAAATTCCTGCTGGATACCAACGCGGTGATCGCCATTCTCAAAGGCGAGCCGGCGATACTGGCCCGTTTGCATGCCTG
GCACCCGGCCGATTTCGGAATCCCCGCGATCGTCGCGCACGAGCTTTACTACGGCGCGTACAAGAGCCAGCGAGCGGCTG
CGAACGTCGCGCGTGTCGACGCGCTGCAGTTCGAGGTCGTATCGTTCGACACCGAGGATGCACAGCATGCGGGTGAAATT
CGCGCGCACCTGATCGCGGCCGGCACGCCGATTGGTCCATACGATGCATTGATCGCAGGGCAGGCGCGTGCGCGGCACCT
GGTGCTGGTCACGCACAACGTCCGCGAATTCGAGCGCGTTCCGCGGCTGCAATTCGAAGACTGGCTAGCCGAACCGAGGG
CAGACTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT189417 WP_003898641.1 NZ_CP069071:1014892-1015071 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT189417 NZ_CP090761:c1887153-1886911 [Burkholderia cenocepacia]
ATGGATACTGCAAAGCTCTTCAAGCATGGCGGCTCGCAGGCTGTCCGGCTACCGAAGGATTTCCGCTTCGATACGGCCGA
GGTTCGCATCCGCCGCCACGGGGCGGCCGTCATTCTCGAGCCGATGCCGCAAGACTGGGCTTGGCTGGCACCGCTGATCG
GTCCGGTGGACGCCGATTTCGAGGCTGCAGCGACCACACAGCCGTCCGCTCAGGAGCGCTCGGGCCTGGACGTGTTCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5