Detailed information of TA system TA189407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 696774..697420
• Replicon: chromosome
• Accession: NZ_CP069071
• Organism: Mycobacterium tuberculosis strain N0136

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: JN142_RS03135
• Protein ID: WP_003403137.1
• Coordinates: 696774..697187 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: JN142_RS03140
• Protein ID: WP_003403139.1
• Coordinates: 697184..697420 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN142_RS03115	692857..694407	+	1551	WP_003403119.1	MlaD family protein	-
JN142_RS03120	694459..694851	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
JN142_RS03125	694848..695105	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
JN142_RS03130	695288..696523	-	1236	WP_003403128.1	ATP-binding protein	-
JN142_RS03135	696774..697187	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN142_RS03140	697184..697420	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
JN142_RS03145	697524..698030	-	507	WP_003900973.1	two component system sensor kinase HK1	-
JN142_RS03150	698144..698674	-	531	WP_003917320.1	two component system sensor kinase HK2	-
JN142_RS03155	698658..699353	-	696	WP_003918099.1	two-component system response regulator TcrA	-
JN142_RS03160	699476..699787	+	312	WP_003403164.1	hypothetical protein	-
JN142_RS03165	699859..700809	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
JN142_RS03170	701050..701634	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
JN142_RS03175	701636..702346	+	711	Protein_629	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T189407 WP_003403137.1 NZ_CP069071:c697187-696774 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T189407 NZ_CP090758:929384-930280 [Burkholderia multivorans]
ATGAGTGTCGTCGACAAACGAGAACGCGACCGCACCGCGGCGTTCGCCACGCTGCTCGAGCGGTTTCCCGAGCAGCGCTG
CGCCGCCGGCGCGGCGGGCACGATCGGTTATCGCGAGGCCGGTGCGCAGCAGGCTGGCGCCGCGCTGCCCGTCGTGCTGC
TGCACGGGATCGGCTCGGGCGCCGCGTCGTGGGTGCGCCAGCTCGACGCGCTCGGCGCTTCGCGCCGCGTGCTCGCGTGG
GACGCGCCCGGCTACGGCGCATCGACGCCGGTGCACGGCGCATCGCCGTCGGCCGTCGATTATGCGGCGTCGCTCGACGC
GTGGCTCGACGCGCTCGGCATCGCGCGCTGCGTGCTCGTCGGGCATTCGCTCGGCGCGATCGTCGCGGGCGGCATGGTGC
GCGTGAAGCCCGAGCGGATCGCGGGGCTGCTGCTGATTTCGCCGGCCGGCGGTTACGGCAGCGCGCCGGCCGAGACGCGC
GCGTCGCGCCGCGACGCGCGGCTCGCGATGCTCGCGGAACTCGGGCCTGCCGGGCTCGCCGCGCAGCGCAGCGGCAACAT
GCTGTCGGGCTTCGCGACCGAAGACGCGCGCGCATGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCGC
AGGCCACGCATCTGCTCGCCAATGCCGATCTCGCGGCCGATCTCGCCGGGTTCCGCGGCCGCACGGCGGTGGCCGTGGGC
GCGAACGACGCGATTACGCCGCCCGCGGCGTGCGAACGCATCGCGGCGGCCGCCGGCGTCGGACTGCAGGTGATTCCGCA
GGCCGGCCACGCGGGCTACGTCGAGGCGCCCGACGTCTATTCGGCCTTGATCGACGCGTTCTGCCGGCAGTGCGACATCG
AGCGGAGGGCGGCATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT189407 WP_003403139.1 NZ_CP069071:c697420-697184 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT189407 NZ_CP090758:930277-931131 [Burkholderia multivorans]
ATGAGCGAACCGTTGCAGAGCGCGGCCGAACCTGCGAAGGCCGAAGCGAGCTACGTCGTGCCCGGCCTCGAGCGCGGGCT
GCGGATTCTCGCCGAATTCTCGCCGCGCGAACCGGTACTCGGAGCGCCGGAGCTGTCGAAGCGGCTCGGCATTCCGCGCA
CGACCGTATTCCGTCTGCTGCAGACGCTGGAATCGCTCGGCTTTCTCGAGCGCGCCGACAAGGACCGCAACTACAAGCTC
GGCATCGCGGTGCTGCGGCTCGGCTTCGAATATCTGAGCTCGCTCGAGCTGACCGATCTCGGCCTGCCCGTGATCGAAAG
CCTGCGCGATGCGACCGGCTTCACGACGCACATCGTGATCCGCGACGGCCGCGACGTCGTGTTCGTTGCGAAGGCGCAGA
GCGAGGCGCCCGTGTTCAGTTCGATTCGCGTGAACGTCGGTACGCGGCTGCCCGCGCACGCGACGACGCACGGTCACGTG
CTGATGGGCGACCTGTCGCTGGACGAGCTGCGCGCGCTGTATCCGGAAGGCACGCTGCCGCGGATGACGAACGCGACGCC
GGACACGGTCGACGCGCTGTACACCATGATCCGCGAGGATGCGCTGCGCGGATACGGCATCAGCAATTCGTCGTTCGAGC
GCGGCATTTCGGTCGTCACCGCGCCGGTACGCAACGACACGCAGAAGATCGTCGCCTGCATCACGGTGACGGTGCCGCGC
CCGGAGATCGACGCGACGCTGATCGCCGACGGGCTGATCGACAAGGTGCGGCGCGCGGCGGCCGAACTGTCGCGGCGGCT
CAATTACCGCTCGGACGACGAACACACGTTTTTGAAGGCTTTGGGACTCCGATGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89