Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3118451..3119021 Replicon chromosome
Accession NZ_CP069070
Organism Mycobacterium tuberculosis strain N1283

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JN143_RS14810 Protein ID WP_003414166.1
Coordinates 3118451..3118807 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JN143_RS14815 Protein ID WP_003901465.1
Coordinates 3118791..3119021 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN143_RS14790 3113903..3115591 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
JN143_RS14795 3115595..3115921 - 327 WP_003414157.1 hypothetical protein -
JN143_RS14800 3116094..3116681 + 588 WP_003914429.1 DUF3558 family protein -
JN143_RS14805 3116700..3118349 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
JN143_RS14810 3118451..3118807 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JN143_RS14815 3118791..3119021 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
JN143_RS14820 3119064..3120107 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
JN143_RS14825 3120106..3120573 + 468 WP_031714166.1 DUF1778 domain-containing protein -
JN143_RS14830 3120749..3121003 - 255 WP_003917684.1 hypothetical protein -
JN143_RS14835 3121151..3121555 + 405 WP_079020684.1 hypothetical protein -
JN143_RS14840 3121552..3121743 + 192 WP_003414184.1 hypothetical protein -
JN143_RS14845 3121960..3122220 + 261 Protein_2930 transposase -
JN143_RS14850 3123330..3123515 + 186 Protein_2931 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T189389 WP_003414166.1 NZ_CP069070:c3118807-3118451 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T189389 NZ_CP090751:68467-68817 [Burkholderia multivorans]
ATGGTGAGGCGGGTCAAGTTTGAGCGGGGCGACATCGTGCGCGTGAGCCTGAACCCGACCATCGGGCGTGAACAGCAGGG
CGATTTCCGTCCCGCGCTCGTGTTGTCTCCGGCCGCGTTCAATGCGTTGGGTGTAGCGCTCGTTGCGCCGATTACTCAAG
GAGGGGAGTTTGCGCGCTTCGCAGGATTCGCCGTGCCGCTCTCAGGTTCGGGTACCGAGACGCAGGGCGTGGCCCTGGTG
AACATGGTGCGTATGCTCGATCTGGAAGCGCGCGGTGCACGCAAGATCGAGCGCGCCCCGGCGGAGGTCGTTGAAGATGC
GCTCGCTCGGCTGCAGACGATAATCGAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT189389 WP_003901465.1 NZ_CP069070:c3119021-3118791 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT189389 NZ_CP090751:68222-68473 [Burkholderia multivorans]
ATGGAACTCAAAATTCAGAAATGGGGCAATAGTGCTGCGGTTCGTCTGCCCGCAGTGTTGCTTGAGCAGATGCATGCCTC
TGTGGGCGGCTCTCTTAACGCGGACGTCCGCCCTGACGGTGTTTTGCTGACCCCTGCACGCCGCAAGTACGCACTGGAAG
ATCTAGTCGCTCAATGCGATGTGAAAGCTCCTGTTCCTGCCGACATGGCTGCATGGGGCGAGGTCAAGCCGGTGGGGCGT
GAAGTATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References