Detailed information of TA system TA189378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-Xre
• Location: 2444708..2445606
• Replicon: chromosome
• Accession: NZ_CP069070
• Organism: Mycobacterium tuberculosis strain N1283

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: JN143_RS11485
• Protein ID: WP_003410001.1
• Coordinates: 2445046..2445606 (+)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: -
• Locus tag: JN143_RS11480
• Protein ID: WP_031718015.1
• Coordinates: 2444708..2445049 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN143_RS11445	2440361..2440717	+	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-
JN143_RS11450	2440770..2441042	+	273	WP_003410017.1	DUF1490 family protein	-
JN143_RS11455	2441039..2443354	+	2316	WP_031582628.1	cation transporter ATPase CptG	-
JN143_RS11460	2443454..2443702	+	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
JN143_RS11465	2443696..2444040	+	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
JN143_RS11470	2444129..2444464	+	336	WP_003410009.1	dehydrogenase	-
JN143_RS11475	2444365..2444622	-	258	WP_003410006.1	hypothetical protein	-
JN143_RS11480	2444708..2445049	+	342	WP_031718015.1	DUF2384 domain-containing protein	Antitoxin
JN143_RS11485	2445046..2445606	+	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
JN143_RS11490	2446126..2446665	-	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
JN143_RS11495	2446891..2447319	-	429	WP_003409992.1	cellulose-binding protein	-
JN143_RS11500	2447735..2448334	-	600	WP_003409989.1	L-lysine exporter	-
JN143_RS11505	2448443..2449354	+	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
JN143_RS11510	2449439..2449669	-	231	WP_031714219.1	DUF167 domain-containing protein	-
JN143_RS11515	2449784..2450437	+	654	WP_003409976.1	cutinase Cfp21	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T189378 WP_003410001.1 NZ_CP069070:2445046-2445606 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T189378 NZ_CP090747:c3411589-3411188 [Burkholderia multivorans]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTATCTGATGAAGAACCAGCCGGAGCAGGTCGCGCGGCGTTTCGC
GCAGTGCTACACGGGGGACGTCGTCATGTCGGCGATCACCTATGCGGAGCTCGAGTACGGCGTGACGCTATGCGCCAATC
CGGCCCGCGAACGCCGCAATCTCGCCGCGCTGATCGAGGATATCCCGGTGGCGCCGTTCGACGGAGCGGCTGCGCAGGCA
TACGGCCCCATTCGAGAGGCGACGCGCGAACGCAAGAAGGATCATCTCGACAAGCTGATCGGCGCGCACGCCGTATCGCT
CGACGTCGTGCTGGTGACCAACAACGAGCGTGATTTCGCGAGTTATCCGGGCTTGAGGCTGGAGAACTGGCTGATCGGCT
AG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12520.39 Da
• Isoelectric Point: 4.8359

>AT189378 WP_031718015.1 NZ_CP069070:2444708-2445049 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEMLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT189378 NZ_CP090747:c3411825-3411589 [Burkholderia multivorans]
ATGCATACCACCCGGGTCTTCCGGAATGGCAACTCGCAGGCGGTGCGGATTCCTGCGGATCTTGCTTATGAGCGCAGCGA
TATCGAACTCGAAATCGAGCGTGTCGGTGACGAAATTCGCATCCGGCCTGCGCGGCGGCCGCTGACCGGCGTGCTCGGCA
AGTTCGCGAAATTCGGGCCCGATTTCATGGCCGAGGGGCGCGGCGATCAGGAACAGGCCGAGCGCGAGGGTTGGTGA

Structures

Toxin

• PDB: 6FKG