Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2443454..2444040 Replicon chromosome
Accession NZ_CP069070
Organism Mycobacterium tuberculosis strain N1283

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag JN143_RS11465 Protein ID WP_003410010.1
Coordinates 2443696..2444040 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag JN143_RS11460 Protein ID WP_003410014.1
Coordinates 2443454..2443702 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN143_RS11440 2439437..2440204 - 768 WP_003410019.1 hemerythrin domain-containing protein -
JN143_RS11445 2440361..2440717 + 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
JN143_RS11450 2440770..2441042 + 273 WP_003410017.1 DUF1490 family protein -
JN143_RS11455 2441039..2443354 + 2316 WP_031582628.1 cation transporter ATPase CptG -
JN143_RS11460 2443454..2443702 + 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
JN143_RS11465 2443696..2444040 + 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
JN143_RS11470 2444129..2444464 + 336 WP_003410009.1 dehydrogenase -
JN143_RS11475 2444365..2444622 - 258 WP_003410006.1 hypothetical protein -
JN143_RS11480 2444708..2445049 + 342 WP_031718015.1 DUF2384 domain-containing protein -
JN143_RS11485 2445046..2445606 + 561 WP_003410001.1 RES family NAD+ phosphorylase -
JN143_RS11490 2446126..2446665 - 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
JN143_RS11495 2446891..2447319 - 429 WP_003409992.1 cellulose-binding protein -
JN143_RS11500 2447735..2448334 - 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T189377 WP_003410010.1 NZ_CP069070:2443696-2444040 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T189377 NZ_CP090747:c1013509-1013132 [Burkholderia multivorans]
ATGGGTATAAATTCATCGATGGTTCCGTACACCAAACCGCTCTACTGGCTCGGCTCGGCACGCAAGGACTTGAAAGCGAT
GCCCGAAGCGGTACAGGACACGTTCGGCTATGCGCTGTATCTGGCCCAGACGGGAGGAAAGCACGATCAGGCCAAGCCGT
TGAGGGGTTTCGGATCGGCCGGCGTACTCGAGGTCGTGGAGTCGGAGGACAGCGGCACCTACCGGGCGGTCTATACGGTG
AAACTCGGTGGCGCCGTCTATGTGCTGCACTGTTTCCAGAAGAAATCGTGCCGCGGTATCGCTACGCCGAAGCCGGACAT
CGAACTGGTTCGCGCGCGCCTGAGCGCCGCCGAAGCACACGCAAATGGAGAAAAGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT189377 WP_003410014.1 NZ_CP069070:2443454-2443702 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT189377 NZ_CP090747:c1013132-1012809 [Burkholderia multivorans]
ATGATCGAAATCGAACGGGGCAGCGGCAACGTCTACGCGGATCTTGGCACGCCCGACGCCGAAGAAATGCGCGTCAAGGC
TCAACTCGCCGCCAAGATCGCGGAGATCATCAAGACTCGCAAATGGACGCAGCAGCACGCAGCACGATTGCTCGGCATGA
CGCAACCGAAGCTATCGAACATGCTGCGCGGGCAGTTTCGCGGCGTAAGCGAAGCGAAGATGCTCGACTGTCTCGCCAAG
CTCGGGCGCGATGTACAGATCGTCGTAGGACCGGACCGTCATTCGGAAACCGAAGGACACATCGAGGTCGTGTTCGCTGC
CTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References