Detailed information of TA system TA189376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2419675..2420301
• Replicon: chromosome
• Accession: NZ_CP069070
• Organism: Mycobacterium tuberculosis strain N1283

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JN143_RS11360
• Protein ID: WP_003410075.1
• Coordinates: 2419675..2420073 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: JN143_RS11365
• Protein ID: WP_003911750.1
• Coordinates: 2420074..2420301 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN143_RS11330	2415275..2416530	+	1256	Protein_2235	HNH endonuclease signature motif containing protein	-
JN143_RS11335	2416658..2417248	-	591	WP_003899131.1	IS110 family transposase	-
JN143_RS11340	2417202..2417822	-	621	WP_003410086.1	IS110 family transposase	-
JN143_RS11345	2417910..2418089	+	180	Protein_2238	hypothetical protein	-
JN143_RS11350	2418526..2418960	-	435	WP_003914358.1	DUF1398 domain-containing protein	-
JN143_RS11355	2419061..2419492	+	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
JN143_RS11360	2419675..2420073	-	399	WP_003410075.1	PIN domain nuclease	Toxin
JN143_RS11365	2420074..2420301	-	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN143_RS11370	2420560..2421729	+	1170	WP_003899126.1	ATP-binding protein	-
JN143_RS11375	2421918..2422262	+	345	WP_003410065.1	ferredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T189376 WP_003410075.1 NZ_CP069070:c2420073-2419675 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T189376 NZ_CP090746:c3322876-3322475 [Burkholderia multivorans]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTATCTGATGAAGAACCAGCCGGAGCAGGTCGCGCGGCGTTTCGC
GCAGTGCTACACGGGGGACGTCGTCATGTCGGCGATCACCTATGCGGAACTCGAGTACGGCGTGACGCTATGCACCAATC
CTGCCCGCGAACGCCGCAATCTCGCCGCGCTGATCGAGGATATCCCGGTGGCGCCGTTCGACGTTGCGGCTGCGCAGGCA
TACGGCCCCATTCGAGAGGCGACGCGCGAACGCAAGAAGGATCATCTCGACAAGCTGATCGGCGCGCACGCCGTATCGCT
CGACGTCGTGCTGGTGACCAACAACGAGCGTGATTTCGCGAGTTATCCGGGCTTGAGGCTGGAGAACTGGCTGATCGGCT
AG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT189376 WP_003911750.1 NZ_CP069070:c2420301-2420074 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT189376 NZ_CP090746:c3323112-3322876 [Burkholderia multivorans]
ATGCATACCACCCGGGTCTTCCGGAATGGCAACTCGCAGGCGGTGCGGATTCCTGCGGATCTTGCTTATGAGCGCAGCGA
TATCGAACTCGAAATCGAGCGTGTCGGTGACGAAATTCGCATCCGGCCTGCGCGGCGGCCGCTGACCGGCGTGCTCGGCA
AGTTCGCGAAATTCGGGCCCGATTTCATGGCCGAGGGGCGCGGCGATCAGGAACAGGCCGAGCGCGAGGGTTGGTGA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8