Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2351881..2352517 Replicon chromosome
Accession NZ_CP069070
Organism Mycobacterium tuberculosis strain N1283

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag JN143_RS11070 Protein ID WP_003410654.1
Coordinates 2351881..2352291 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag JN143_RS11075 Protein ID WP_003410651.1
Coordinates 2352284..2352517 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN143_RS11050 2347478..2348701 + 1224 WP_003901321.1 class I SAM-dependent methyltransferase -
JN143_RS11055 2348647..2350173 - 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
JN143_RS11060 2350170..2350796 - 627 WP_003410658.1 precorrin-8X methylmutase -
JN143_RS11065 2350806..2351897 - 1092 WP_003900454.1 precorrin-3B synthase -
JN143_RS11070 2351881..2352291 - 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
JN143_RS11075 2352284..2352517 - 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
JN143_RS11080 2352595..2356179 + 3585 WP_003410648.1 cobaltochelatase subunit CobN -
JN143_RS11085 2356263..2356667 + 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
JN143_RS11090 2356668..2357060 - 393 WP_229297931.1 metal ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T189374 WP_003410654.1 NZ_CP069070:c2352291-2351881 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T189374 NZ_CP090746:2030773-2031066 [Burkholderia multivorans]
ATGGTTACTTTGCTGCGATCCACGGAGTTCAACGATTGGCTTGCCAACCTGCGCGATCAGCGCGGCAAGGCCCGAATCGC
AGCCCGCCTGATCAGCGCGCAACTCGGCAACTTCGGCGAGTACCGTGTGCTCGACGAAGGGGTCTGCGAACTGAAGATCG
ATTTCGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGTGGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCA
ACGCAACGGAAGGACATCAAGCCCGTGCAGATGGCGCGCGAACTGAAGGAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT189374 WP_003410651.1 NZ_CP069070:c2352517-2352284 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT189374 NZ_CP090746:2031070-2031354 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGACGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCACGGTCGCGCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References