Detailed information of TA system TA189370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1815063..1815703
• Replicon: chromosome
• Accession: NZ_CP069070
• Organism: Mycobacterium tuberculosis strain N1283

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JN143_RS08545
• Protein ID: WP_003412970.1
• Coordinates: 1815284..1815703 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JN143_RS08540
• Protein ID: WP_003412975.1
• Coordinates: 1815063..1815287 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN143_RS08520	1810680..1811243	+	564	WP_003412989.1	elongation factor P	-
JN143_RS08525	1811246..1811716	+	471	WP_003899364.1	transcription antitermination factor NusB	-
JN143_RS08530	1811716..1812117	+	402	WP_003412981.1	hypothetical protein	-
JN143_RS08535	1812189..1815032	+	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN143_RS08540	1815063..1815287	+	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JN143_RS08545	1815284..1815703	+	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN143_RS08550	1815704..1816654	-	951	WP_003911916.1	ERCC4 domain-containing protein	-
JN143_RS08555	1816752..1816958	+	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN143_RS08560	1817299..1818219	+	921	WP_003412965.1	restriction endonuclease	-
JN143_RS08565	1818254..1818655	-	402	WP_003412963.1	PIN domain-containing protein	-
JN143_RS08570	1818652..1818879	-	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
JN143_RS08575	1819396..1820118	+	723	WP_003412957.1	DUF1906 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189370 WP_003412970.1 NZ_CP069070:1815284-1815703 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189370 NZ_CP090745:c1429692-1428796 [Burkholderia multivorans]
ATGAGTGTCGTCGACAAACGAGAACGCGACCGCACCGCGGCGTTCGCCACGCTGCTCGAGCGGTTTCCCGAGCAGCGCTG
CGCCGCCGGCGCGGCGGGCACGATCGGTTATCGCGAGGCCGGTGCGCAGCAGGCTGGCGCCGCGCTGCCCGTCGTGCTGC
TGCACGGGATCGGCTCGGGCGCCGCGTCGTGGGTGCGCCAGCTCGACGCGCTCGGCGCTTCGCGCCGCGTGCTCGCGTGG
GACGCGCCCGGCTACGGCGCATCGACGCCGGTGCACGGCGCATCGCCGTCGGCCGTCGATTATGCGGCGTCGCTCGACGC
GTGGCTCGACGCGCTCGGCATCGCGCGCTGCGTGCTCGTCGGGCATTCGCTCGGCGCGATCGTCGCGGGCGGCATGGTGC
GCGTGAAGCCCGAGCGGATCGCGGGGCTGCTGCTGATTTCGCCGGCCGGCGGTTACGGCAGCGCGCCGGCCGAGACGCGC
GCGTCGCGCCGCGACGCGCGGCTCGCGATGCTCGCGGAACTCGGGCCTGCCGGGCTCGCCGCGCAGCGCAGCGGCAACAT
GCTGTCGGGCTTCGCGACCGAAGACGCGCGCGCATGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCGC
AGGCCACGCATCTGCTCGCCAATGCCGATCTCGCGGCCGATCTCGCCGGGTTCCGCGGCCGCACGGCGGTGGCCGTGGGC
GCGAACGACGCGATTACGCCGCCCGCGGCGTGCGAACGCATCGCGGCGGCCGCCGGCGTCGGACTGCAGGTGATTCCGCA
GGCCGGCCACGCGGGCTACGTCGAGGCGCCCGACGTCTATTCGGCCTTGATCGACGCGTTCTGCCGGCAGTGCGACATCG
AGCGGAGGGCGGCATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT189370 WP_003412975.1 NZ_CP069070:1815063-1815287 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189370 NZ_CP090745:c1428799-1427945 [Burkholderia multivorans]
ATGAGCGAACCGTTGCAGAGCGCGGCCGAACCTGCGAAGGCCGAAGCGAGCTACGTCGTGCCCGGCCTCGAGCGCGGGCT
GCGGATTCTCGCCGAATTCTCGCCGCGCGAACCGGTACTCGGAGCGCCGGAGCTGTCGAAGCGGCTCGGCATTCCGCGCA
CGACCGTATTCCGTCTGCTGCAGACGCTGGAATCGCTCGGCTTTCTCGAGCGCGCCGACAAGGACCGCAACTACAAGCTC
GGCATCGCGGTGCTGCGGCTCGGCTTCGAATATCTGAGCTCGCTCGAGCTGACCGATCTCGGCCTGCCCGTGATCGAAAG
CCTGCGCGATGCGACCGGCTTCACGACGCACATCGTGATCCGCGACGGCCGCGACGTCGTGTTCGTTGCGAAGGCGCAGA
GCGAGGCGCCCGTGTTCAGTTCGATTCGCGTGAACGTCGGTACGCGGCTGCCCGCGCACGCGACGACGCACGGTCACGTG
CTGATGGGCGACCTGTCGCTGGACGAGCTGCGCGCGCTGTATCCGGAAGGCACGCTGCCGCGGATGACGAACGCGACGCC
GGACACGGTCGACGCGCTGTACACCATGATCCGCGAGGATGCGCTGCGCGGATACGGCATCAGCAATTCGTCGTTCGAGC
GCGGCATTTCGGTCGTCACCGCGCCGGTACGCAACGACACGCAGAAGATCGTCGCCTGCATCACGGTGACGGTGCCGCGC
CCGGAGATCGACGCGACGCTGATCGCCGACGGGCTGATCGACAAGGTGCGGCGCGCGGCGGCCGAACTGTCGCGGCGGCT
CAATTACCGCTCGGACGACGAACACACGTTTTTGAAGGCTTTGGGACTCCGATGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09