Detailed information of TA system TA189369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1800451..1801160
• Replicon: chromosome
• Accession: NZ_CP069070
• Organism: Mycobacterium tuberculosis strain N1283

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ26
• Locus tag: JN143_RS08455
• Protein ID: WP_003413164.1
• Coordinates: 1800747..1801160 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: JN143_RS08450
• Protein ID: WP_003413167.1
• Coordinates: 1800451..1800708 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN143_RS08410	1795544..1796056	+	513	WP_003413197.1	Holliday junction resolvase RuvX	-
JN143_RS08415	1796049..1797302	+	1254	WP_003413196.1	endolytic transglycosylase MltG	-
JN143_RS08420	1797299..1798108	+	810	WP_003413193.1	shikimate dehydrogenase	-
JN143_RS08425	1798120..1798539	+	420	WP_003413190.1	A24 family peptidase	-
JN143_RS08430	1798950..1799195	+	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JN143_RS08435	1799192..1799587	+	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JN143_RS08440	1799687..1800061	+	375	WP_003413177.1	hypothetical protein	-
JN143_RS08445	1800077..1800454	-	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
JN143_RS08450	1800451..1800708	-	258	WP_003413167.1	CopG family transcriptional regulator	Antitoxin
JN143_RS08455	1800747..1801160	-	414	WP_003413164.1	PIN domain nuclease	Toxin
JN143_RS08460	1801253..1801531	-	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
JN143_RS08465	1801528..1802190	-	663	WP_003900848.1	LppA family lipoprotein	-
JN143_RS08470	1802187..1802846	-	660	WP_003900847.1	LppA family lipoprotein	-
JN143_RS08475	1802843..1803502	-	660	WP_003900846.1	LppA family lipoprotein	-
JN143_RS08480	1803629..1804840	-	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
JN143_RS08485	1805136..1805450	-	315	WP_009937839.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15045.00 Da
• Isoelectric Point: 5.4673

>T189369 WP_003413164.1 NZ_CP069070:c1801160-1800747 [Mycobacterium tuberculosis]
MVFCVDTSAWHHAARPEVARRWLAALSADQIGICDHVRLEILYSANSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTIVWHYDENYDRVAAITGQPTEWIVPRGTL

Nucleotide

• Length: 414 bp

>T189369 NZ_CP090745:1131615-1131914 [Burkholderia multivorans]
ATGCCGGTTAAGGCGCGCACGGTACGCCTGACGCCGCTGGCGGAGGCCGACCTCGAGGCCATCTGGTCTTACACGTACAA
GCGCTGGTCGCTCGACCAGGCTGAGCGCTATATCGGCGAGCTGAGCGCGGCGTTCGAGCGGCTGGCGCGCCGGGAGTCGG
TAGGGTGGCCGAGCCGCGCGGGCGGCAACTATTCGCGGTACCTGGTCGGTTCGCACGTGGTGTTCTATCGGGAAACGACC
GAAACCCTTGACGTCATTCGCGTGCTGCACCAGCGGATGGATGTCGACCGACATCTGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT189369 WP_003413167.1 NZ_CP069070:c1800708-1800451 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT189369 NZ_CP090745:1131380-1131625 [Burkholderia multivorans]
ATGGCACGAAATACATCGATCTCGCTGGGCGATCATCTGACCGAATTTGTCGACATGCAGGTCGCGTCCGGCCGCTACGG
CTCGGCTAGCGATGTCGTGCGCGCGGGCCTGCGCCTGCTCGAGACACAGGAAACCGAGCTGCGGGCGCTGCAGGAAGCGT
TGAAGGCGGGCGAGGCATCGGGCACACCGGCTCCGTTCGACAGCCAGGCGTTCCTGGCCCGGATGCGGGCCAAGCATGCC
GGTTAA

Structures

Toxin

• AlphaFold DB: G0TQ26

Antitoxin

• AlphaFold DB: A0A829C9W5