Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1798950..1799587 Replicon chromosome
Accession NZ_CP069070
Organism Mycobacterium tuberculosis strain N1283

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN143_RS08435 Protein ID WP_003413180.1
Coordinates 1799192..1799587 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN143_RS08430 Protein ID WP_003413183.1
Coordinates 1798950..1799195 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN143_RS08410 1795544..1796056 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
JN143_RS08415 1796049..1797302 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN143_RS08420 1797299..1798108 + 810 WP_003413193.1 shikimate dehydrogenase -
JN143_RS08425 1798120..1798539 + 420 WP_003413190.1 A24 family peptidase -
JN143_RS08430 1798950..1799195 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN143_RS08435 1799192..1799587 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN143_RS08440 1799687..1800061 + 375 WP_003413177.1 hypothetical protein -
JN143_RS08445 1800077..1800454 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN143_RS08450 1800451..1800708 - 258 WP_003413167.1 CopG family transcriptional regulator -
JN143_RS08455 1800747..1801160 - 414 WP_003413164.1 PIN domain nuclease -
JN143_RS08460 1801253..1801531 - 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN143_RS08465 1801528..1802190 - 663 WP_003900848.1 LppA family lipoprotein -
JN143_RS08470 1802187..1802846 - 660 WP_003900847.1 LppA family lipoprotein -
JN143_RS08475 1802843..1803502 - 660 WP_003900846.1 LppA family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189367 WP_003413180.1 NZ_CP069070:1799192-1799587 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189367 NZ_CP090745:c405581-405264 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189367 WP_003413183.1 NZ_CP069070:1798950-1799195 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189367 NZ_CP090745:c405260-404961 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
TTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTCAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References