Detailed information of TA system TA189366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1738533..1739247
• Replicon: chromosome
• Accession: NZ_CP069070
• Organism: Mycobacterium tuberculosis strain N1283

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: JN143_RS08170
• Protein ID: WP_003413460.1
• Coordinates: 1738533..1738973 (-)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: JN143_RS08175
• Protein ID: WP_003413456.1
• Coordinates: 1738960..1739247 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN143_RS08140	1733669..1734271	-	603	Protein_1606	PPE family protein	-
JN143_RS08145	1734445..1735119	-	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-
JN143_RS08150	1735247..1736146	+	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
JN143_RS08155	1736175..1737020	+	846	WP_003413466.1	acyl-CoA thioesterase II	-
JN143_RS08160	1737028..1737624	+	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
JN143_RS08165	1737757..1738512	+	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
JN143_RS08170	1738533..1738973	-	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN143_RS08175	1738960..1739247	-	288	WP_003413456.1	antitoxin VapB41	Antitoxin
JN143_RS08180	1739358..1740929	-	1572	WP_003899392.1	polyamine aminopropyltransferase	-
JN143_RS08185	1740926..1741384	-	459	WP_003413451.1	DUF350 domain-containing protein	-
JN143_RS08190	1741409..1741840	-	432	WP_003899390.1	DUF4247 domain-containing protein	-
JN143_RS08195	1741837..1742331	-	495	WP_003413444.1	DUF2617 family protein	-
JN143_RS08200	1742342..1742962	-	621	WP_003413441.1	DUF4178 domain-containing protein	-
JN143_RS08205	1743179..1743583	-	405	WP_003899389.1	type II toxin-antitoxin system VapC family toxin	-
JN143_RS08210	1743580..1743825	-	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T189366 WP_003413460.1 NZ_CP069070:c1738973-1738533 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T189366 NZ_CP090745:c405260-404961 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
TTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTCAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT189366 WP_003413456.1 NZ_CP069070:c1739247-1738960 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT189366 NZ_CP090745:c405581-405264 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8