Detailed information of TA system TA189356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1016181..1016800
• Replicon: chromosome
• Accession: NZ_CP069070
• Organism: Mycobacterium tuberculosis strain N1283

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN143_RS04815
• Protein ID: WP_003404726.1
• Coordinates: 1016366..1016800 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: JN143_RS04810
• Protein ID: WP_003898641.1
• Coordinates: 1016181..1016360 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN143_RS04795	1011654..1013234	+	1581	WP_003909313.1	serine hydrolase	-
JN143_RS04800	1013231..1015624	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
JN143_RS04805	1015897..1016055	+	159	WP_003404720.1	hypothetical protein	-
JN143_RS04810	1016181..1016360	+	180	WP_003898641.1	antitoxin	Antitoxin
JN143_RS04815	1016366..1016800	+	435	WP_003404726.1	SRPBCC family protein	Toxin
JN143_RS04820	1016898..1017671	+	774	WP_003404735.1	VOC family protein	-
JN143_RS04825	1017736..1018185	+	450	WP_003404738.1	hypothetical protein	-
JN143_RS04830	1018320..1018550	-	231	WP_003898642.1	hypothetical protein	-
JN143_RS04835	1018717..1020225	-	1509	WP_031714135.1	carotenoid oxygenase family protein	-
JN143_RS04840	1020227..1021465	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T189356 WP_003404726.1 NZ_CP069070:1016366-1016800 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T189356 NZ_CP090740:2437313-2438239 [Burkholderia multivorans]
GTGAGCAAACGGAACACCGCCGCCTCGGTCCGCGCGCGACTGCTCGTCAAGGCCCGCACCGACAAGCAGGACTTCAACCT
CGTCCTCACCCGCTACGCCTTGGAGCGGCTCCTGTATCGCCTGAGCGTCTCGGCTCATGCCGACCATTTTCTCCTGAAGG
GGGCGTTACTGTTCGACCTGTGGTTCGACATCCCGCATCGTCCGACACGCGACGCCGACCTGCTGGGCTTCGGCTCGGCC
GAGATTCCGCATGTCGAGGCGGCGTTTCGGGAGATCTGTGCGGTGGAACTGGACGACGGCATACGCTTCCAAGCCGACTC
GGTACACGCGGAGGAGATCCGCAAGGAAGCCAACTACTCCGGTGTCCGGGTCACGCTCCTCGGACTGTTGGACGGTGCCC
GATGCCACGTGCAGGTCGACGTGGGTTTCGGTGACGCCGTGACACCCGGCCCCGAGGCTGTCGATTACCCGGTGATGCTG
CCGGACATGCCTGCCCCGAAGCTGCGTGCCTACCCCCGCTACACGGTCGTCGCCGAGAAACTGGAAGCGCTGGTGTCACT
CGGCATCGCCAATAGCCGCATGAAGGATTACTTCGACCTCTGGATCTTGTCGCGCCACAGCGAATTCGACGGCGCGCTCC
TGTCCAAGGCGATCCACGCCACCTTCGAGCGCCGCAGGACGCCGCTGCCGGACGGCGTCCCTTTCGGCTTAAGTGACGAA
TTCGCTCAGGATCGGCAGAAGCAGACCCAATGGCAGGCATTCTTGAGGAAGAATGCTTTGGTCGAGCTTCAGCTTTCCGA
GGTCATTGCCGGGCTGCGTGCGTTCCTGTCATTCCCGCTGGACGCGCTACGGCAAGCTGCCGCTTTTCCGCTGGCGTGGC
GTGCAGATAGCGGGTGGACGCCGAGCACCGAGGAATCTGGCCGATGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT189356 WP_003898641.1 NZ_CP069070:1016181-1016360 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT189356 NZ_CP090740:2436717-2437316 [Burkholderia multivorans]
ATGGACAGTTCACACCAGAGCATTCTGGAACTTGCCGCCCAGCGCGGCCTTATTCGCCCACGTGATCTCAATGAGCGCGG
GCTACCCACTGTCGCTCTCACGCGGCTGGTGCGGCAAGGGCTGCTCCAGCGCGTGGGACGGGGCCTGTATGCGATTCCGG
ACCGGCCCGTCTCGGAGCACGGCGCGCTGGCCGAAGTGGCACGCAAAAACCCGCAGGCCATCGTCTGCCTGCTGTCGGCA
CTGCGCGTGCACGAACTCACCACACAGTCGCCATTCGAGGTCTGGCTGGCGATCCCGAACAAGGCGCGCGCGCCAAAGAT
GGATTATCCCCCGCTGCGCATCGTGCGCTTCTCTGGCGCAGCGCTGACGGAGGGGATTGAGGAACACTCCGTCGATGGTG
TGCCGGTGCGCGTGACCAACGTCGCCCGAACGGTGGCCGACTGCTTCAAATTCCGCAACAAGATCGGCCTCGATGTCGCG
CTGGAGGCGCTGCAGGAAGCCTGGCGCGCCAAGCGTGTCAGCATGGATGAGCTGTGGCGCTTTGCTGAGCTGTGCCGAGT
GGCCAATGTGATGCGGCCCTACCTGGAGAGTCTGACGTGA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5