Detailed information of TA system TA189343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 642728..643404
• Replicon: chromosome
• Accession: NZ_CP069070
• Organism: Mycobacterium tuberculosis strain N1283

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: JN143_RS02900
• Protein ID: WP_003898500.1
• Coordinates: 642728..643141 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: JN143_RS02905
• Protein ID: WP_003402915.1
• Coordinates: 643138..643404 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN143_RS02870	638073..638375	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
JN143_RS02875	638435..638713	-	279	WP_003402901.1	hypothetical protein	-
JN143_RS02880	638710..639963	-	1254	WP_003402904.1	inorganic phosphate transporter	-
JN143_RS02885	640083..640469	-	387	WP_003402907.1	VOC family protein	-
JN143_RS02890	640532..641416	-	885	WP_003402909.1	SDR family oxidoreductase	-
JN143_RS02895	641512..642414	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
JN143_RS02900	642728..643141	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN143_RS02905	643138..643404	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JN143_RS02910	643596..645311	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
JN143_RS02915	645389..646993	+	1605	WP_003402920.1	amidohydrolase	-
JN143_RS02920	646990..647970	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T189343 WP_003898500.1 NZ_CP069070:c643141-642728 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T189343 NZ_CP090737:c3556196-3555795 [Burkholderia cepacia]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTATTTGATGAAGCATCAGCCGGAACAGGTCGCGAAACGATTTGC
CCAGTGCTACACGGGGGACGTCGTGATGTCGGCGATTACGTACGCCGAGCTCGAATACGGCGTGGCCGCGTGTGCGAATG
CCGTCCGGGAGCGACGCAACCTTGCCGACCTGATCGAGGATATTCCGGTTGCGCCGTTCGACGCGGCGGCCGCGCAAGCG
TACGGCCCTGTTCGCGAAGCGACCCGCGAGCGGAAGAGGGATCATCTCGACAAACTGATCGCGGCGCACGCGGTGTCGCT
CGATGTCGTGCTCGTGACCAACAACGAGCGGGATTTCGCGAGTTACCCGGGGTTGCGGCTGGAGAACTGGCTGAACGACT
AG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT189343 WP_003402915.1 NZ_CP069070:c643404-643138 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT189343 NZ_CP090737:c3556432-3556196 [Burkholderia cepacia]
ATGCATACCACGAGGGTGTTCCGGAACGGCAACTCGCAGGCCGTACGGATTCCGGCGGATCTTGCCTACGAGCGCAGCGA
TATCGAGCTCGAAATCGAACGGATCGGCGACGAAATCCGTATTCGTCCCGTGCGACGGCCGTTGGCTGGCGTGCTCGAGA
AGTTCGCGAAATTCGCACCGGATTTCATGGCTGAAGGGCGTGGCGACCAGGAGCAGGCCGACCGCGAGGGTTTGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0