Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3195581..3196268 Replicon chromosome
Accession NZ_CP069069
Organism Mycobacterium tuberculosis strain N1176

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag JN144_RS15245 Protein ID WP_003414624.1
Coordinates 3195825..3196268 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag JN144_RS15240 Protein ID WP_003414620.1
Coordinates 3195581..3195838 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN144_RS15220 3190901..3191755 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
JN144_RS15225 3191811..3192974 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
JN144_RS15230 3192991..3194205 - 1215 WP_003414610.1 zinc metalloprotease Rip -
JN144_RS15235 3194213..3195454 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
JN144_RS15240 3195581..3195838 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
JN144_RS15245 3195825..3196268 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
JN144_RS15250 3196348..3197010 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
JN144_RS15255 3197106..3197297 + 192 WP_003909769.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
JN144_RS15260 3197629..3199377 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
JN144_RS15265 3199473..3200054 + 582 WP_003414644.1 fasciclin domain-containing protein -
JN144_RS15270 3200154..3200420 + 267 WP_031667416.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T189332 WP_003414624.1 NZ_CP069069:3195825-3196268 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T189332 NZ_CP090733:2566044-2566970 [Burkholderia multivorans]
GTGAGCAAACGGAACACCGCCGCCTCGGTCCGCGCGCGACTGCTCGTCAAGGCCCGCACCGACAAGCAGGACTTCAACCT
CGTCCTCACCCGCTACGCCTTGGAGCGGCTCCTGTATCGCCTGAGCGTCTCGGCTCATGCCGACCATTTTCTCCTGAAGG
GGGCGTTACTGTTCGACCTGTGGTTCGACATCCCGCATCGTCCGACACGCGACGCCGACCTGCTGGGCTTCGGCTCGGCC
GAGATTCCGCATGTCGAGGCGGCGTTTCGGGAGATCTGTGCGGTGGAACTGGACGACGGCATACGCTTCCAAGCCGACTC
GGTACACGCGGAGGAGATCCGCAAGGAAGCCAACTACTCCGGTGTCCGGGTCACGCTCCTCGGACTGTTGGACGGTGCCC
GATGCCACGTGCAGGTCGACGTGGGTTTCGGTGACGCCGTGACACCCGGCCCCGAGGCTGTCGATTACCCGGTGATGCTG
CCGGACATGCCTGCCCCGAAGCTGCGTGCCTACCCCCGCTACACGGTCGTCGCCGAGAAACTGGAAGCGCTGGTGTCACT
CGGCATCGCCAATAGCCGCATGAAGGATTACTTCGACCTCTGGATCTTGTCGCGCCACAGCGAATTCGACGGCGCGCTCC
TGTCCAAGGCGATCCACGCCACCTTCGAGCGCCGCAGGACGCCGCTGCCGGACGGCGTCCCTTTCGGCTTAAGTGACGAA
TTCGCTCAGGATCGGCAGAAGCAGACCCAATGGCAGGCATTCTTGAGGAAGAATGCTTTGGTCGAGCTTCAGCTTTCCGA
GGTCATTGCCGGGCTGCGTGCGTTCCTGTCATTCCCGCTGGACGCGCTACGGCAAGCTGCCGCTTTTCCGCTGGCGTGGC
GTGCAGATAGCGGGTGGACGCCGAGCACCGAGGAATCTGGCCGATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT189332 WP_003414620.1 NZ_CP069069:3195581-3195838 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT189332 NZ_CP090733:2565448-2566047 [Burkholderia multivorans]
ATGGACAGTTCACACCAGAGCATTCTGGAACTTGCCGCCCAGCGCGGCCTTATTCGCCCACGTGATCTCAATGAGCGCGG
GCTACCCACTGTCGCTCTCACGCGGCTGGTGCGGCAAGGGCTGCTCCAGCGCGTGGGACGGGGCCTGTATGCGATTCCGG
ACCGGCCCGTCTCGGAGCACGGCGCGCTGGCCGAAGTGGCACGCAAAAACCCGCAGGCCATCGTCTGCCTGCTGTCGGCA
CTGCGCGTGCACGAACTCACCACACAGTCGCCATTCGAGGTCTGGCTGGCGATCCCGAACAAGGCGCGCGCGCCAAAGAT
GGATTATCCCCCGCTGCGCATCGTGCGCTTCTCTGGCGCAGCGCTGACGGAGGGGATTGAGGAACACTCCGTCGATGGTG
TGCCGGTGCGCGTGACCAACGTCGCCCGAACGGTGGCCGACTGCTTCAAATTCCGCAACAAGATCGGCCTCGATGTCGCG
CTGGAGGCGCTGCAGGAAGCCTGGCGCGCCAAGCGTGTCAGCATGGATGAGCTGTGGCGCTTTGCTGAGCTGTGCCGAGT
GGCCAATGTGATGCGGCCCTACCTGGAGAGTCTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References