Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3149226..3149830 Replicon chromosome
Accession NZ_CP069069
Organism Mycobacterium tuberculosis strain N1176

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag JN144_RS15020 Protein ID WP_003414492.1
Coordinates 3149226..3149618 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag JN144_RS15025 Protein ID WP_003414495.1
Coordinates 3149615..3149830 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN144_RS14990 3144376..3145164 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
JN144_RS14995 3145498..3146043 - 546 WP_003904931.1 DUF1802 family protein -
JN144_RS15000 3146315..3147199 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
JN144_RS15005 3147202..3148089 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
JN144_RS15010 3148394..3148939 - 546 WP_003899500.1 DUF1802 family protein -
JN144_RS15015 3148936..3149205 - 270 WP_003414489.1 DUF2277 family protein -
JN144_RS15020 3149226..3149618 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
JN144_RS15025 3149615..3149830 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JN144_RS15030 3149877..3150626 + 750 WP_003414497.1 enoyl-CoA hydratase -
JN144_RS15035 3150705..3151787 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
JN144_RS15040 3151780..3153090 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
JN144_RS15045 3153093..3153920 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
JN144_RS15050 3153917..3154828 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T189330 WP_003414492.1 NZ_CP069069:c3149618-3149226 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T189330 NZ_CP090733:c84745-84344 [Burkholderia multivorans]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTATCTGATGAAGAACCAGCCGGAGCAGGTCGCGCGGCGTTTCGC
GCAGTGCTACACGGGGGACGTCGTCATGTCGGCGGTCACCTATGCGGAGCTCGAGTACGGCGTGACGCTATGCGCCAATC
CGGCCCGCGAACGCCGCAATCTCGCCGCGCTGATCGAGGATATCCCGGTGGCGCCGTTCGACGGAGCGGCTGCGCAGGCA
TACGGCCCCATTCGAGAGGCGACGCGCGAACGCAAGAAGGATCATCTCGACAAGCTGATCGGCGCGCACGCCGTATCGCT
CGACGTCGTGCTGGTGACCAACAACGAGCGTGATTTCGCGAGTTATCCGGGCTTGAGGCTGGAGAACTGGCTGATCGGCT
AG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT189330 WP_003414495.1 NZ_CP069069:c3149830-3149615 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT189330 NZ_CP090733:c84981-84745 [Burkholderia multivorans]
ATGCATACCACCCGGGTCTTCCGGAATGGCAACTCGCAGGCGGTGCGGATTCCTGCGGATCTTGCTTATGAGCGCAGCGA
TATCGAACTCGAAATCGAGCGTGTCGGTGACGAAATTCGCATCCGGCCTGCGCGGCGGCAGCTGACCGGCGTGCTCGGCA
AGTTCGCGAAATTCGGGCCCGATTTCATGGCCGAGGGGCGCGGCGATCAGGAACAGGCCGAGCGCGAGGGTTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References