Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3122093..3122663 Replicon chromosome
Accession NZ_CP069069
Organism Mycobacterium tuberculosis strain N1176

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JN144_RS14875 Protein ID WP_003414166.1
Coordinates 3122093..3122449 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JN144_RS14880 Protein ID WP_003901465.1
Coordinates 3122433..3122663 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN144_RS14855 3117545..3119233 - 1689 Protein_2932 alpha/beta hydrolase family protein -
JN144_RS14860 3119237..3119563 - 327 WP_003414157.1 hypothetical protein -
JN144_RS14865 3119736..3120323 + 588 WP_031667424.1 DUF3558 family protein -
JN144_RS14870 3120342..3121991 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
JN144_RS14875 3122093..3122449 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JN144_RS14880 3122433..3122663 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
JN144_RS14885 3122706..3123749 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
JN144_RS14890 3123748..3124215 + 468 WP_003414177.1 DUF1778 domain-containing protein -
JN144_RS14895 3124391..3124645 - 255 WP_003917684.1 hypothetical protein -
JN144_RS14900 3124793..3125197 + 405 WP_003414181.1 hypothetical protein -
JN144_RS14905 3125194..3125385 + 192 WP_003414184.1 hypothetical protein -
JN144_RS14910 3125602..3125862 + 261 Protein_2943 transposase -
JN144_RS14915 3126972..3127229 + 258 WP_003899489.1 hypothetical protein -
JN144_RS14920 3127334..3127645 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T189328 WP_003414166.1 NZ_CP069069:c3122449-3122093 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T189328 NZ_CP090732:2676190-2676507 [Burkholderia cepacia]
ATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTTTGCCGGCCTGCGGGATCGCGT
CGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACTGGAAATCCGCGGGCTCGCCGG
TCGTCGAAATGCGGATCGACCACGGCCCCGGTTATCGCGTCTACTACGTCCGGCGCGGAACGATCTGGGTCATCCTGCTC
TGCGGCGGCGATAAATCGACGCAACAGGCCGACATACGCGCCGCGCACGCGATGCTCGCGCACCTCGACATGGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT189328 WP_003901465.1 NZ_CP069069:c3122663-3122433 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT189328 NZ_CP090732:2676511-2676810 [Burkholderia cepacia]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAGACCGAAGACGACATGGCCGACTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCACGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCGAGCTTCGGCACC
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCGCAAAAGCGCACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References